Immunologist & Microbiologist 👩🏻🔬🔬🧬 | Postdoc at NIAID @NIH | PhD from @Penn @BrodskyIgor lab | BS from @JohnsHopkins | Mexicana 🇲🇽 | Views my own
Washington, DC
Joined March 2020
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Really excited to share a short tweetorial of some of my work as a grad student in the @BrodskyIgor lab @pennvet, now in press at @ScienceAdvances! 🧵👇science.org/doi/10.1126/scia…
Today was surreal! Thank you to everyone who came in person and virtually, especially my awesome committee @metaBailism @kelliejurado @SunnyShinLab @taabaman and @BrodskyIgor for being such an amazing thesis advisor!
Congratulations Dr. @RonitSchwartz99 @CAMBUpenn on an amazing thesis defense today!
science.org/doi/10.1126/scia…
Thanks to thesis committee chair @metaBailism, committee members @SunnyShinLab @taabaman @kelliejurado & Shin & Brodsky lab for all the advice and support along the way!
Ronit Schwartz, PhD retweeted
Ronit @RonitSchwartz99 in the @BrodskyIgor lab defends her thesis on Monday!
Ronit Schwartz, PhD retweeted
Congratulations Ronit! Awesome study working out sequential cell death pathway activation during Yersinia Pestis infection. We are currently using this paper in the lab as a blueprint for some other work!
Really excited to share a short tweetorial of some of my work as a grad student in the @BrodskyIgor lab @pennvet, now in press at @ScienceAdvances! 🧵👇science.org/doi/10.1126/scia…
Ronit Schwartz, PhD retweeted
Check out the latest study led by @RonitSchwartz99 from the @BrodskyIgor lab. The talented @TheBourneImmuno helped unravel the intricate response to Yersinia, which activates sequentially and spatially distinct death complexes!
science.org/doi/10.1126/scia…
Ronit Schwartz, PhD retweeted
Amazing work by the incredibly talented @RonitSchwartz99 and @BrodskyIgor. Many of these cell death pathways are interconnected and happy we could help with this important mechanistic insight. Check out the excellent tweetorial below 👇🏾
Really excited to share a short tweetorial of some of my work as a grad student in the @BrodskyIgor lab @pennvet, now in press at @ScienceAdvances! 🧵👇science.org/doi/10.1126/scia…
Ronit Schwartz, PhD retweeted
#WeekendRead! #LiveForever! @RonitSchwartz99 @BrodskyIgor &co show @ScienceAdvances that the pathogen Yersinia by blocking immune signaling kinases activates proapoptic caspase-8 that in turn activate caspase-1 to drive death independently of #inflammasome science.org/doi/10.1126/scia…
Ronit Schwartz, PhD retweeted
Want to know how caspases work together? Brodsky et al explain how caspase-8 and caspase-1 collaborate during bacterial infections. Significant implications for cell death and inflammation biology. Kudos to all authors.
@BrodskyIgor @RonitSchwartz99
science.org/doi/10.1126/scia…
Ronit Schwartz, PhD retweeted
So excited to have this story out and so fun to work on this project led by the amazing @RonitSchwartz99! Thanks to our collaborators @taabaman @pscottlab Olaf Gross @BeaHerrm Winslow Yost @TheBourneImmuno and others!
Really excited to share a short tweetorial of some of my work as a grad student in the @BrodskyIgor lab @pennvet, now in press at @ScienceAdvances! 🧵👇science.org/doi/10.1126/scia…
Huge thanks all our co-authors and collaborators- Winslow Yost, @BeaHerrm, @TheBourneImmuno, Daniel Sorobetea, @christinakgo, Benedikt Saller, Olaf Groß, @pscottlab, Anthony Rongvaux, Doug Green, Bart Tummers, @monack_lab, and @taabaman!
Overall, our study reveals that spatially & temporally distinct but functionally-linked death complexes mediate cell death/IL-1 release in response to blockade of TAK1/IKK & highlight the multiple ways cells can engage to produce IL-1b to defend against microbial infection.
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However, these ASC puncta don’t co-localize with active casp8 in the cell, and robust activation of casp8 occurs well before assembly of ASC puncta or detectable casp1 processing.
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In WT cells, casp8-dependent casp1 activity leads to GSDMD processing, inducing secondary activation of NLRP3->ASC->casp1->IL-1b. Even though ASC is not required for Yp-induced cell death, ASC puncta assemble in Yp-infected cells, responsible for IL-1b processing.
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We additionally found that casp1 catalytic activity was necessary for its own processing downstream of YopJ activity, implying that casp8 initiates, and casp1 amplifies, its own activation in response to Yp. In the casp8DA/DA BMDMs, casp1 cleavage depends on necroptosis.
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In this study, we show that casp8 cleaves casp1 directly in 293T cells, & this requires both casp8 auto-processing & catalytic activity. Surprisingly however, in BMDMs from casp8DA/DA mice, where casp8 can’t be auto processed, Yp still induces casp1 processing and cell death!
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Our group and others previously reported that Yersinia (Yp) YopJ is a potent inhibitor of innate immune signaling components, and this inhibition triggers cell death in mouse macrophages. However, we still had many questions about how macrophages actually die during infection.
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Had so much fun presenting our work at @ImmunologyAAI #AAI2024 in Chicago- thanks to everyone who came to my talk and stopped by my poster! And thanks to @BrodskyIgor for your guidance and encouragement!
Ronit Schwartz, PhD retweeted
Catch me at IMMUNOLOGY2024 on Sunday at 10am presenting a really cool collaborative project I’ve been lucky to be a part of between @BrodskyIgor , @SunnyShinLab , and @taabaman labs at @Penn on noncanonical inflammasome assembly in response to cytosolic LPS! @ImmunologyAAI
Very excited to be speaking at IMMUNOLOGY2024™ this Saturday May 4th about my work from the @BrodskyIgor lab on cell death responses to Yersinia infection!
Ronit Schwartz, PhD retweeted
Our and @BrodskyIgor’s labs witnessing the solar eclipse (amidst lots of ☁️)!
