
ALT The effect of UVR (UVB: 280-315 nm) across developmental age (dpf) of clownfish (A. ocellaris) embryos. Embryos were exposed to either control (no UVR; pink colour bar) or UVR levels representing the within-depth range of A. ocellaris: 6 m (low UVR treatment; 80 µW m2; green colour bar) or 2 m (high UVR treatment; 280 µW m2; blue colour bar) (Braun et al., 2016). UVR exposure occurred for 2 h daily from when eggs were laid (0 dpf) until hatched at 8-9 dpf. On 2 and 5 dpf, individual embryos were collected for (A) mass (mg; n=15 per age per treatment group), and (B) yolk sac volume (mm3; n=15 per age per treatment group). (C) DNA damage (concentration of CPD) only was measurable in embryos exposed to high UVR treatment only (n=13 measured in 2 dpf embryos, and n=8 measured in 5 dpf embryos). The experiment was replicated three times per treatment using two different parent pairs. In all three panels, predicted Bayesian posterior distributions are shown with symbols and error bars repres

ALT Total biodiversity (N = 787) captured by environmental DNA (eDNA) metabarcoding of water samples collected at eight sites across the Gold Coast Broadwater Estuary, represented as (A) total number of taxa based on amplified sequence variants (ASVs) for different taxonomic subgroups and (B) taxonomic classifications of the ASVs at different ranks. Subgroups were ordered according to count from highest to lowest. Silhouettes depicting the taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).

ALT Principal component analysis (PCA) comparing the similarities in presence/absence of (A) eukaryotic micro-organisms, (B) fish, (C) invertebrates, (D) other, (E) photosynthetic organisms, (F) single-celled micro-organisms, and (G) all taxa (i.e., all amplicon sequence variants or ASVs) along the Gold Coast Broadwater Estuary based on environmental DNA (eDNA) water samples collected at eight sites and an estuarine water quality index (WQI). Silhouettes depicting the taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).

ALT Total biodiversity (N = 787) captured by environmental DNA (eDNA) metabarcoding of water samples collected at eight sites across the Gold Coast Broadwater Estuary, represented as (A) total number of taxa based on amplified sequence variants (ASVs) for different taxonomic subgroups and (B) taxonomic classifications of the ASVs at different ranks. Subgroups were ordered according to count from highest to lowest. Silhouettes depicting the taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).

ALT Number of new taxa (N = 137) based on the classification of the unique amplified sequence variants (ASVs) detected in the Gold Coast Broadwater Estuary from environmental DNA (eDNA) water samples collected at eight sites. Taxa were deemed “new” if there were no existing occurrence records in the Atlas of Living Australia (ALA) online biodiversity repository based on an exact match to our assigned taxonomic name (accessed February 2024). Subgroups were ordered according to count from highest to lowest. Silhouettes depicting taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).

ALT Number of new taxa (N = 137) based on the classification of the unique amplified sequence variants (ASVs) detected in the Gold Coast Broadwater Estuary from environmental DNA (eDNA) water samples collected at eight sites. Taxa were deemed “new” if there were no existing occurrence records in the Atlas of Living Australia (ALA) online biodiversity repository based on an exact match to our assigned taxonomic name (accessed February 2024). Subgroups were ordered according to count from highest to lowest. Silhouettes depicting taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).

ALT Principal component analysis (PCA) comparing the similarities in presence/absence of (A) eukaryotic micro-organisms, (B) fish, (C) invertebrates, (D) other, (E) photosynthetic organisms, (F) single-celled micro-organisms, and (G) all taxa (i.e., all amplicon sequence variants or ASVs) along the Gold Coast Broadwater Estuary based on environmental DNA (eDNA) water samples collected at eight sites and an estuarine water quality index (WQI). Silhouettes depicting the taxonomic subgroups were sourced from the package rphylopic (Gearty and Jones, 2023).






















