Alex Shaw

Training for rapid DDNS sequencing of poliovirus at the Institut Pasteur de Madagascar with trainees from Côte d'Ivoire, CAR, Madagascar and Algeria @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Dr Nuno Faria opening at CADDE Workshop on Portable Metagenomics for Pathogen Surveillance 2023 @CaddeProject @MRC_Outbreak

And to finish off, the lab have performed near-whole genome sequencing of poliovirus in single reads using native barcoding. Great work! @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Rapid analysis of DDNS poliovirus data using @AineToole 's Poliovirus Investigation Resource Automating Nanopore Haplotype Analysis (PIRANHA) software running in ARTIFICE- from RNA to sequences in 2.5 days. @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Great progress on the first two DDNS sequencing runs with the Omani CHPL #polio team! @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outbreak @polionanopore

Training in direct detection and nanopore sequencing (DDNS) for rapid #polio outbreak detection underway with the Central Public Health Laboratories in Oman. @OmaniMOH @VaccineEpi @NIBSC_MHRA @MRC_Outreak @polionanopore

Just arrived for #GrandChallenges 2022! Will be presenting poliovirus and NPEV collaborative surveillance work between @VaccineEpi @labgenpath #INRB @NIBSC_MHRA @AineToole and Andrew Rambaut. @MRC_Outbreak

Filming now in session for our Direct Detection of poliovirus by Nanopore Sequencing (DDNS) protocol training videos @VaccineEpi @MRC_Outbreak @gatesfoundation with @AlistairMB

Our group has an opening for a Research Associate focused on the evolution and epidemiology of poliovirus: imperial.ac.uk/jobs/descript…

First trial of our remote training for direct detection of poliovirus from stool by nanopore sequencing today, with the Institut National de Recherche Biomédicale in DRC @nanopore @inrb_kinshasa

Polio sequencing trip episode 3: This time it'll work flawlessly. Off to Pakistan we go.

Anyone inferring more sequencing issues from my lack of tweets would be correct. Two weeks of troubleshooting, results due in the <distant> future. Real science is hard.. if this were a movie we would have obliterated several invading alien species with that amount of effort.

One near heart attack later, after it looked like another failed protocol, we might actually have some polio genomes to sequence. Hopefully I won't succumb to library-prep related trauma first.

Better do one of those selfies (as my work experience kids spent the entire week doing). Spent mins trying to get both the suitcases in and make my face less round. Achieved neither. Prob need an emoji: 🐠

We'll begin with the valiant effort to stuff everything into a suitcase again. Definitely going to work this time. I should probably add a hashtag too.. er.. #Suitcase (I think that's how this works- I'm sure @alis_njc will correct me otherwise)

Hmm.. how to phrase this in the overwhelmingly positive Twitter manner.. "Delighted to be heading back to Vellore to give polio sequencing from poo another try thanks to the protocol going wrong last time 😁". Possibly should use more emojis...

And we're finally on to sequencing- 50 multiplexed faecal samples being tested for presence of Sabin strain polio on one flow cell.