Hamidu Tei Mohammed

ALT Electron micrograph of a bacterium attacked and infested by phages

ALT Agar from different suppliers induces msrP under anaerobic conditions. (A) Suppliers, catalog (reference) numbers, and batch numbers of the different agar powders used in this study. We assigned a number (from 7 to 10) to each agar reference to facilitate the reading of panels B to D. (B) Colony morphology of wild-type (MG1655) and ?msrP (CH380) strains streaked onto LB agarose (?) or LB agar. The plates were incubated at 37�C under anaerobic conditions for 48 h. Bar?=?1?mm. (C and D) After overnight anaerobic growth at 37�C in LB supplemented or not with agar, the expression of the PhiuH-lacZ reporter (strain CH184) was measured using ?-galactosidase assays (C), and the production of MsrP was monitored by immunoblot analysis (D). (C) Error bars indicate standard deviations (n?=?3). ***, P?<?0.001 (Dunnett?s multiple-comparison test). (D) Anti-RecA was used as a loading control.