.@David_Cameron our BBC's world-renowned&trusted because it's independent & invested in. #BackTheBBC 38d.gs/protect-our-bbc-Dont trash it!
#HPLC Gold rule of LC .... other parameters eg sound of the system, are rarely recorded yet can give important clues to troubleshooting..
#HPLC Gold rule of LC .... Some of the operating parameters eg flow rate & pressure are easy to quantify.....
#HPLC... why HILIC.... IC - high salt concentration? Non charged species? Compatibility with MS & ELSD?
#HPLC-......"pure silica" ... extremely low metal content; best materials for the analysis of bases.
#HPLC-......"pure silica" ... extremely low metal content; best materials for the analysis of bases.
#HPLC-"pure silica" produced via the polymerisation of tetraethylorthosilicate; uniform associated silanol groups are produced .......
#HPLC Remember.. if you are out by 1% in your mobile phase composition.. that can equal a HUGE 5% change in retention time
#HPLC - ...... ENCAPPING is used to reduce the no. of isolated silanols and only 30% of available silanols are capped!!!
#HPLC-ENDCAPPING is performed after bonding C18 onto silica;......
#HPLC-"base deactivated" silica is acid treated to reduce metals which are responsible for increasing the acidity of the silica surface
#HPLC -How to overcome the problem of peak tailing: solution 3 - use PURE silica!
#HPLC -How to overcome the problem of peak tailing: solution 2 - use "base deactivated" silica!
#HPLC -How to overcome the problem of peak tailing: solution 1 - ENDCAPPING!
Recruiting #HPLC current vacancies @hichrom - Scientific Officer / Higher Scientific Officer ow.ly/IToyR
Not-so-new to #HPLC? then Dr John Dolan is running 2 courses 13/14th May, in Manchester: HPLC MD & Validating Methods - there is time!
All the details of the Prof Mel Euerby & Dr John Dolan Hichrom #HPLC courses can be found here. htm ow.ly/ITjP3
