Post Doctoral Fellow at @Queensu | P.h.D in Kinesiology from @McMasterUniversity
Kingston, Ontario
Joined December 2017
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Absolutely thrilled to share our work recently published in NAR molecular medicine on identifying a non-coding RNA (ncRNA) signature associated with skeletal muscle remodelling. Below is a thread of some (but not all 😉) interesting findings academic.oup.com/narmolmed/a…
Jonathan Mcleod retweeted
$50M McCall MacBain Foundation gift propels McMaster’s leadership in helping people live longer in good health news.mcmaster.ca/50m-gift-su…
The wait is finally over! I'm so happy to receive my @binding_broken copies of Malazan. They are incredibly beautiful. Also, my cat really enjoys the box 😂
Jonathan Mcleod retweeted
& note Illumina seq only measures in total 20% of lncRNA & each muscle illumina seq can't reproducibly that 20% (different set each spin of the wheel) as you can see from Jon's plot.
Critically, majority of these ncRNAs are not consistently detected using illumnia bulk-RNA seq, which further supports work published by @tjstokes5 @metapredict @mackinprof onlinelibrary.wiley.com/doi/….
We used 437 resting human skeletal muscle samples in a robust network analysis and found that some of our ncRNA candidates mediate their association with muscle growth via multiple different cell types (e.g., CYTOR is associated with an enriched immune cell network/response).
I just want to say a huge thank you to all the co-authors on this project, none of this work would have been possible without you. @metapredict @mackinprof @ChanghyunLim1 @tjstokes5 @AlyshaDSouza1 @drlaurencs1 @james_mckendry @RobMorton23 @saraoikawa and many others not on X 🙂
Jonathan Mcleod retweeted
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling
Fm @MACleod_JC @ChanghyunLim1 @tjstokes5 @AlyshaDSouza1 @james_mckendry @RobMorton23 @saraoikawa @metapredict @mackinprof
doi.org/10.1093/narmme/ugae0…
Jonathan Mcleod retweeted
Detailed illustration of how modelling of bulk transcriptomic data (Custom Affymetrix HTA & no not random WGCNA) provides information about rare cell types (<1% of bulk)
@MACleod_JC studies long noncoding RNAs during muscle hypertrophy - 90% missed by illumina bulk RNAseq @thermofisher
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling academic.oup.com/narmolmed/a…
Jonathan Mcleod retweeted
Excellent visit to @queensu today, thx to Principal Patrick Deane and the inspiring faculty and students for the great hospitality. Enjoyed learning about dark matter from Art McDonald & the lab tour with @cathleencrudden. Nice to see firsthand the impact of @NSERC_CRSNG funding.
Jonathan Mcleod retweeted
Muscle matters: the effects of medically induced weight loss on skeletal muscle thelancet.com/journals/landi…
Check out our new pre-print (biorxiv.org/content/10.1101/…) where we highlight #ncRNA in human skeletal muscle following supervised exercise training!!
To provide functional context for these ncRNA genes, we used quantitative network modelling (using 437 baseline muscle samples) of protein coding genes and ncRNA genes and used spatial images to identify potential cell-specific locations!
This incredible project could not have been possible without the stellar co-authors! Huge shoutout to the senior authors and my mentors on this project @metapredict @mackinprof