Investigator with the National Human Genome Research Institute, in the NIH. Views are my own.
Bethesda, MD
Joined January 2016
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New from my lab: Ancestral sequence reconstruction analysis indicates the furin-cleavage-site insertion in the SARS-CoV-2 spike gene likely interrupted a codon academic.oup.com/ve/article/…
Meru Sadhu retweeted
New paper in #G3journal from @mongemmmm, @MeruSadhu, and colleagues presents a barcode-based high-throughput method that can track large numbers of independent replicates of a small number of combinatorial genotypes. buff.ly/Q0qgBQz
New paper from my lab! We describe our idea that high-throughput pooled experimental methods – typically used to test thousands of hypotheses at once – also have huge potential to help in “everyday” experiments testing one or a few focused hypotheses. doi.org/10.1093/g3journal/jk…
“High throughput” doesn’t have to mean hard/expensive! At its heart, the difference is that instead of picking a few clones of your transformation, you take all the colonies. And with low sequencing costs and the possibility of pooling sequencing, it will generally be affordable.
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Meru Sadhu retweeted
New preprint from the lab: A maternal-effect toxin-antidote element causes larval arrest in C. elegans, by @sdraljefainc et al. biorxiv.org/content/10.1101/…
Meru Sadhu retweeted
Our obsession with yeast killer toxins was triggered by a collaboration with @MeruSadhu.
Now with @BPSIYork we have a PhD project to delve deeper into toxin effects (and defences) at the biophysical level.
Great opportunity for diverse skillsets. Apply:
tinyurl.com/y877rj3b
Now out in Genome Research! "Highly complete long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity" genome.cshlp.org/content/33/…
Thanks again to Team Genome: Cory A. Weller, Ilya Andreev, Michael Chambers (@greenkidneybean), Morgan Park, Josh Bloom (@joshsbloom), and the NIH Intramural Sequencing Center
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And thanks to Genome Research @genomeresearch and the anonymous reviewers - the entire process was very helpful and improved the manuscript substantially!
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Lab news: Postbac Molly Monge is headed to Weill Cornell’s MD PhD program @TriIMDPhD! Congrats, Molly and Cornell!
Wooo, first paper from the lab! We discovered a yeast defense factor against the killer toxin K28: a previously uncharacterized gene we named KTD1. Check it out! Open access on PNAS: pnas.org/doi/10.1073/pnas.22…
Big congrats to Ilya Andreev, the postbac who fearlessly took on the project! And all the co-authors: Kamilla Laidlaw, Simone Giovanetti, Guillaume Urtecho (@goGUrtecho), Daniel Shriner, Josh Bloom (@JoshSBloom) and Chris MacDonald (@YorkYeast)
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I’m excited to share the latest from my lab, now on the bioRxiv! We combined long-read genome sequencing with QTL mapping to understand how pangenomic variation causes phenotypic diversity in yeast. 🧵 biorxiv.org/content/10.1101/…
Big thanks to “Team Genome”! Cory A. Weller, Ilya Andreev, Michael Chambers (@greenkidneybean), Morgan Park, Josh Bloom (@joshsbloom), and the NIH Intramural Sequencing Center