@SmallThingsLab

Tweets by Rohit Ghai, #PELAGICS European Lakes Sampling Expedition (#GACR EXPRO Excellence Project)

 Budweis, Czech Republic
hbu.cas.cz/en/structure/ame/laboratory-of-microbial-ecology-and-evolution/
Joined August 2018
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Heterotrophic nanoflagellates in lakes ingest 10–20 bacteria/hour, removing roughly 1/3 of all prokaryotes daily but most remain uncultured and unsequenced. We devised a new cultivation strategy to describe one such predator here 🦖🔬 academic.oup.com/ismej/advan… #MicrobialEcology

Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Abstract. Heterotrophic nanoflagellates are the chief agents of bacterivory in the aquatic microbial loop but remain underrepresented in culture collection

academic.oup.com
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But here’s the bombshell: In the same culture we found 2 giant viruses,Tyrannovirus reginensis GV1 & 2 belonging to a viral genus known only from metagenomes. “Unculturable” protists & their giant viruses can be recovered by novel cultivation methods + genomics. Cultures matter!
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We are happy to share our work on the first isolate of the CRY1a lineage,which we baptized Tyrannomonas regina. It is a small and voracious predator (≈3–5 µm)🦖with the smallest genome among cryptomonads (69 Mb).
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The CRY1a lineage is ubiquitous and abundant across global freshwaters. Our CARD-FISH surveys across 84 lakes and 3 lagoons show that CRY1a often makes up >10% of all heterotrophic nanoflagellates. #FreshwaterEcology #ProtistDiversity
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On the biological meaning of the population pangenome dlvr.it/TM98Bv
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SmallThingsLab retweeted
Huiluo Cao 曹慧荦@hlcao29 Jul 2025
On the biological meaning of the population pangenome: Trends in Microbiology cell.com/trends/microbiology…

On the biological meaning of the population pangenome

The prokaryotic pangenome, the full complement of genes within a species, is strikingly large. To understand how ecological forces shape this diversity, it is useful to examine the variable gene pool...

cell.com
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🧬 Interested in aquatic micro, nutrient cycling, river-ocean interfaces I’m seeking a postdoc candidate to apply w/ me for the 2026 Walder Biota Fellowship ($70k+/yr 2 yrs, $20k/yr research). Details: More info 👉 biotaawards.org/2026-applica… Our Lab 👉 theaquaticmicrobiologylab.co…

2026 Application — Biota Awards

biotaawards.org
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🧬Interested in protist genomics and viruses ? 🦠Do take a look at the latest from @SmallThingsLab: Lineage-specific expansions of polinton-like viruses in photosynthetic cryptophytes microbiomejournal.biomedcent… 🚀Great work by @paul_bulzu and @AqueladeTroia ! #PELAGICS #GACR

Lineage-specific expansions of polinton-like viruses in photosynthetic cryptophytes

Microbiome - Polinton-like viruses (PLVs) are diverse eukaryotic DNA viral elements (14–40 kb) that often undergo significant expansion within protist genomes through repeated insertion...

link.springer.com
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SmallThingsLab retweeted
APB_Lab@apb_lab26 May 2025
Postdoc position: “Eutrophication and AAP bacteria: changes in the contribution of AAP bacteria to microbial dynamics and carbon cycle in response to trophic status”. Apply here: mbu.cas.cz/kariera/volne-poz…
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🚨 PhD Opportunity Alert! 🚨 Passionate about virus-protist interactions? Join us to explore Giant Viruses & Polintons ! 🧬 #PhDPosition #Microbiology #Virology #PhDlife Deadline: 31 March 2025 jobs.bc.cas.cz/en/detail/233 @paul_bulzu @SmallThingsLab
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SmallThingsLab retweeted
Ramiro Logares@ramalok9 Jan 2025
Exciting news for aquatic microbial ecologists! The 18th SAME meeting will take place in Barcelona (Sept 28–Oct 3, 2025) 🧬🌊 Interested? Pls, share your details here: forms.gle/XUTfGqq3tpgL7zqT8—it only takes a minute! Spread the word & save the date! 🗓️ @EMM_Barcelona @ICMCSIC
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🚨 PhD Opportunity Alert! 🚨 Passionate about virus-protist interactions? Join us to explore Giant Viruses & Polintons ! 🧬 #PhDPosition #Microbiology #Virology #PhDlife Deadline: 31 March 2025 jobs.bc.cas.cz/en/detail/233 @paul_bulzu @SmallThingsLab
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finally out! congratulations to @DinaBkl and the entire #PELAGICS team! Global freshwater distribution of Telonemia protists academic.oup.com/ismej/artic…

Global freshwater distribution of Telonemia protists

Abstract. Telonemia are one of the oldest identified marine protists that for most part of their history have been recognized as a distinct incertae sedis

academic.oup.com
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My poster on bacteriophages in maternal-infant metagenomes (PS1.02.035) will still be available to view this morning at #ISME19 @ISME_microbes in Cape Town, South Africa! Come check it out!
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Excited to share the published version of my recent work on protist dynamics and microbial interactions: doi.org/10.1186/s40793-024-0…

Integrating depth-dependent protist dynamics and microbial interactions in spring succession of a...

Environmental Microbiome - Protists are essential contributors to eukaryotic diversity and exert profound influence on carbon fluxes and energy transfer in freshwaters. Despite their significance,...

link.springer.com
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great to catch up with old friends back in alicante @frovalera and ana. thanks for the wonderful time!
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PostDoc position!! 📢Are you interested in parasitic interactions among aquatic microbes? Do you like working with phages? We have a 1 year postdoc position with the possibility for extension! Read more and apply here: bionomics-mmlab.com/open-pos…

Bionomics Lab - Open positions

Would you like to join us?! If we have open PhD or PostDoc positions available in the Bionomics Lab we post them here as well. We always welcome master students and visiting researchers interested in...

bionomics-mmlab.com
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biorxiv.org/content/10.1101/… 130 different clones from the same Pelagibacter species at a single point in time and space in the Western Med

Diversity of the O-chain biosynthetic cluster in the Pelagibacterales

In this study, we have used single-amplified genomes (SAGs) and long-read metagenomics to examine the intrapopulation diversity of the O-chain polysaccharide biosynthesis cluster (OBC) in marine...

biorxiv.org
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Big congratulations to PhD @cristian_vi_al 🎉🎊🥳 @apb_lab
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Check out our latest contribution to AAP ecology. Many thanks to all that made it possible @apb_lab @IzabelaMujakic @CarlotaRGazulla @jasonnwoodhouse @hpgrossart @KasiaPiwosz @SmallThingsLab @VKavagutti and more! link.springer.com/article/10…

Phenology and ecological role of aerobic anoxygenic phototrophs in freshwaters

Microbiome - Aerobic anoxygenic phototrophic (AAP) bacteria are heterotrophic bacteria that supply their metabolism with light energy harvested by bacteriochlorophyll-a-containing reaction centers....

link.springer.com
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