@SpruijtLab

Our lab uses coacervate based materials to investigate protocells, synthetic cells, and biomolecular condensates. Also on Bluesky *Operated by the lab

 Radboud University, Nijmegen
spruijtlab.com/
Joined February 2019
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56Media
Curious about the open questions in the field of liquid-liquid phase separation? Check out our latest paper in Communications Chemistry for a discussion of current insights and open questions. Read here: nature.com/articles/s42004-0…

Open questions on liquid–liquid phase separation

Communications Chemistry - Liquid-liquid phase separation (LLPS) underlies the formation of intracellular membraneless compartments in biology and may have played a role in the formation of...

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Still looking 👀 for a suitable candidate. Please retweet
Dimova Lab now at @DimovaLab.bsky.social@DimovaLab4 Nov 2024
📢 Open PhD position as part of #MSCA network of 17 students @ComeInCell Interested in exploring membrane stability/repair & #condensates ? Check out & apply: 👉bit.ly/4fd8HRU The project will include secondments at @SaricLab, @MarquesLab & @NatureComms
https://doi.org/10.1038/s41586-023-06726-w

ALT https://doi.org/10.1038/s41586-023-06726-w

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Curious how biocondensates localize ions? We found that condensates selectively bind and localize both chaotropic anions and kosmotropic cations. This alters the condensate composition, interface potential and RNA duplex stability inside. By @IrisSmokers biorxiv.org/content/10.1101/…

Selective ion binding and uptake shape the microenvironment of biomolecular condensates

Biomolecular condensates modulate various ion-dependent cellular processes and can regulate subcellular ion distributions by selective uptake of ions. However, the molecular grammar governing...

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Selective ion binding and uptake shape the microenvironment of biomolecular condensates doi.org/10.1101/2024.12.24.6…
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We are #hiring! For more information about our 17 #PhDposition and our #membrane and #membranecondensate projects , please check our website comeincell.org/projects/
ComeInCell@ComeInCell1 Nov 2024
🚀 Exciting News! Today marks the official launch of the ComeInCell MSCA Doctoral Network! 🎉 Join us as we embark on a journey to advance cellular research and innovation. Check the press release: mpikg.mpg.de/6845600/launch-…

ComeInCell Launches:A European Doctoral Network to Train the Next Generation of Cell Scientists 

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We're thrilled to announce an open PhD position on exploring controlling condensate membrane attachment and exocytosis! Collaborate across Europe in the ComeInCell EU network. Apply now! Details: comeincell.org/ & ru.nl/en/working-at/job-oppo…
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SpruijtLab retweeted
Claudia@ClaudiaBonfio3 Nov 2024
Ever wondered when coacervates appeared on early Earth and what they looked like? Think no more and have a look at our new preprint! Incredibly proud of @karinakinuyo and @mihoubi_nina for their incredible work, dedication and patience! 🤩 chemrxiv.org/engage/chemrxiv…

Compositional and functional diversity of minimal primitive coacervates in a nucleic acid-peptide...

The RNA-peptide world hypothesis postulates the early co-evolution of RNA and peptides, leading to the emergence of non-enzymatic RNA replication and peptide synthesis. Although nucleotides and amino...

chemrxiv.org
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#ChemSystemsChem Phase-separated Droplets Can Direct the Kinetics of Chemical Reactions Including Polymerization, Self-replication and Oscillating Networks (Evan Spruijt and co-workers) #openaccess onlinelibrary.wiley.com/doi/…

Phase‐Separated Droplets Can Direct the Kinetics of Chemical Reactions Including Polymerization,...

Using a numerical model, we study how phase-separated compartments can influence chemical reactions. We show how the volume ratio (R) and partition coefficient (Kp) affect reaction rates in bimolec...

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Our latest preprint is now live on bioRxiv! We demonstrate how to control α-synuclein adsorption, desorption, and aggregation at charged biomolecular condensate interfaces. Check it out here: doi.org/10.1101/2024.10.20.6…
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Check out a new @NaturePortfolio article collection on Liquid–liquid phase separation in cells. nature.com/collections/iabce… As part of the many great articles, you will find a Review from the @SpruijtLab published recently in Nat. Rev. Chem. nature.com/articles/s41570-0…

Liquid–liquid phase separation in cells

Multivalent interactions between biological molecules result in the formation of biomolecular condensates, a phenomenon known as Liquid–liquid phase ...

nature.com
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Want to measure the volume fraction of biocondensates or coacervates? We present easy and accurate methods, with which we found that upon destabilization, condensates can either shrink or swell, giving insight in condensate volume regulation in cells. chemrxiv.org/engage/chemrxiv…
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The role of biomolecular condensates in protein aggregation nature.com/articles/s41570-0… A review by Brent Visser, Wojciech Lipiński & Evan Spruijt from @SpruijtLab @Radboud_Uni
The graphical abstract from the article highlighting the various effects that can affect liquid to solid transitions.

ALT The graphical abstract from the article highlighting the various effects that can affect liquid to solid transitions.

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We are thrilled to announce that our latest review on the role of biomolecular condensates in protein aggregation is now published in @NatRevChem! Many thanks to the reviewers for their valuable suggestions and feedback. Open access here: nature.com/articles/s41570-0…
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Coacervates can accelerate (bio)chemical reactions and direct their outcome. In our new review by @IrisSmokers we detail how their unique properties allow them to function as catalytic microcompartments at the origins of life. Have a look here: pubs.acs.org/doi/10.1021/acs…

How Droplets Can Accelerate ReactionsCoacervate Protocells as Catalytic Microcompartments

Conspectus. Coacervates are droplets formed by liquid–liquid phase separation (LLPS) and are often used as model protocells–primitive cell-like compartment

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How Droplets Can Accelerate Reactions─Coacervate Protocells as Catalytic Microcompartments dlvr.it/T9Cx6J

How Droplets Can Accelerate Reactions─Coacervate Protocells as Catalytic Microcompartments - PubMed

Coacervates are droplets formed by liquid-liquid phase separation (LLPS) and are often used as model protocells-primitive cell-like compartments that could have aided the emergence of life. Their...

pubmed.ncbi.nlm.nih.gov
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SpruijtLab retweeted
Huck Group@Huckgroup28 Jun 2024
In our new paper, now published in @Nature, we report on the discovery and implementation of a chemical reservoir computer based on the prebiotic formose reaction. Congrats to Mathieu, Thijs, @quentin_duez , @robn_will and Wilhelm!🥳 Read the paper 👇 nature.com/articles/s41586-0…
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The Many-Chemicals Problem of Systems Chemistry by Oliver R. Maguire @oliver_maguire onlinelibrary.wiley.com/doi/…

The Many‐Chemicals Problem of Systems Chemistry

An E. coli cell contains ~2500 different chemicals that form a biochemical system from which a happy living bacterium emerges. A chemist combining together 2500 different chemicals from a laboratory...

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Exciting lab news! How can machine learning help us understand and predict #biomolecular #condensates? Thrilled to start my postdoc in @ProteinMagnus lab with this perspective led by Maria (@mcd_611) in @JMolBiol. Check it out! sciencedirect.com/science/ar… #Biophysics #MolBiol #LLPS
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Over the weekend our publication on studying the surface charge of condensates on a single droplet level was published in @NatureComms! You can read it now here: doi.org/10.1038/s41467-024-4…

Probing the surface charge of condensates using microelectrophoresis

Nature Communications - The surface charge and ζ-potential of biomolecular condensates is key to their interactions with membranes and proteins. Here, the authors developed a method to...

nature.com
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How do coacervates direct the outcome of (bio)chemical reactions? We developed a model to predict their effect on bimolecular, polymerization, self-replication and oscillating reactions, to guide experiments. Exciting results for origins of life & biology! chemrxiv.org/engage/chemrxiv…
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For oscillating reaction networks the periodicity and amplitude of oscillations can be altered by incorporation in a two-phase system, and oscillations can even be ‘rescued’ in concentration regimes where sustained oscillations do not take place in a single-phase system.
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