@YaegerLuke

Postdoc, Laboratory of Kim Lewis, Northeastern University. Natural product antibiotic discovery.

Joined June 2019
  • Tweets176
  • Following327
  • Followers210
5Media
Unveiling the taxonomic diversity and unprecedented biosynthetic treasure of the phylum Myxococcota biorxiv.org/content/10.64898… #biorxiv_micrbio
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Check out some great structural work on AmpG, a PG recycling transporter I think is very cool!
Lori L. Burrows@Dr_Lori_Burrows18 Nov 2024
Just in time for #WAAW, collab with the Strynadka lab ⁦⁦@UBC -⁩ Cryo-EM characterization of the anydromuropeptide permease AmpG central to bacterial fitness and β-lactam antibiotic resistance. ⁦@McMasterIIDR⁩ ⁦@BBS_McMasterU⁩ ⁦#AMR nature.com/articles/s41467-0…

Cryo-EM characterization of the anydromuropeptide permease AmpG central to bacterial fitness and...

Nature Communications - AmpG is an MFS importer of bacterial cell wall fragments. Here, authors present a cryoEM structure and supporting mutagenesis to illuminate AmpG’s essential role in...

nature.com
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Luke Yaeger retweeted
Richard@Richard_E_Lee31 Jul 2024
It is hard to beat nature in the design of antibiotics. In this review, we analyze the sophisticated mechanisms of many antibiotic substances with an eye toward future antibacterial drug discovery. nature.com/articles/s41586-0…

Sophisticated natural products as antibiotics

Nature - This Review examines the diverse strategies utilized by naturally occurring antibiotics and suggests how they have provided, and will in future provide, inspiration for the design of novel...

nature.com
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Read about macrolones, the antibiotics that target the ribosome and DNA gyrase: pubmed.ncbi.nlm.nih.gov/3903…. A great collaboration with the Polikanov lab (UIC) and Liang lab (BIT).
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CRISPRi-Tn-Seq! Great collaboration with @tvanop nature.com/articles/s41564-0…

CRISPRi–TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria

Nature Microbiology - CRISPRi knockdown of essential genes combined with TnSeq of non-essential genes yields a high-throughput method for bacterial genetic interaction mapping.

nature.com
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Our paper on the sequence diversity of the ribosome-targeting apidaecin-like peptides found in the synthetic gene library is out: academic.oup.com/nar/article…

Sequence diversity of apidaecin-like peptides arresting the terminating ribosome

Abstract. The Proline-rich Antimicrobial Peptide (PrAMP) apidaecin (Api) inhibits translation by binding in the ribosomal nascent peptide exit tunnel, trap

academic.oup.com
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Inducible transposon mutagenesis for genome-scale forward genetics biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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If you've induced phages, you may have wondered if you can do the 'opposite' - block entry into lysogeny. You can, using protein synthesis inhibitors! Better still, this yields a potent synergy, functionally turning a temperate phage into a virulent one. journals.asm.org/doi/10.1128…
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I’m stoked to share one of the last stories of my PDF @McMasterIIDR. Found a potential way to prevent infection-associated urinary stones by blocking nickel uptake in K. pneumoniae. Thanks to all who helped make this paper happen @gerryiidr @prehnalab nature.com/articles/s41467-0…

Targeting bacterial nickel transport with aspergillomarasmine A suppresses virulence-associated...

Nature Communications - Aspergillomarasmine A (AMA) chelates metal ions such as Zn2+ and Ni2+, which are essential for the activity of enzymes that are important for virulence of several pathogens....

nature.com
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Happy to share our latest by @YaegerLuke & @Mike_MR_Ranieri - #Pseudomonas aeruginosa biofilm stimulation by sub-MIC #antibiotics! 1/9 nature.com/articles/s41522-0… rdcu.be/dCiT7

A genetic screen identifies a role for oprF in Pseudomonas aeruginosa biofilm stimulation by...

npj Biofilms and Microbiomes - A genetic screen identifies a role for oprF in Pseudomonas aeruginosa biofilm stimulation by subinhibitory antibiotics

nature.com
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Redefining development in Streptomyces venezuelae: integrating exploration into the classical sporulating life cycle @mbiojournal by Evan Shepherdson and Marie Elliot journals.asm.org/doi/10.1128…
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Synthesis of lipid-linked precursors of the bacterial cell wall is governed by a feedback control mechanism in Pseudomonas aeruginosa | Nature Microbiology nature.com/articles/s41564-0…

Synthesis of lipid-linked precursors of the bacterial cell wall is governed by a feedback control...

Nature Microbiology - Peptidoglycan biosynthesis is regulated by a feedback mechanism preventing the pathway from sequestering essential lipid carriers used to assemble and transport surface glycan...

nature.com
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pGpG-signaling regulates virulence and global transcriptomic targets in Erwinia amylovora biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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Awesome new paper from @whiteleylab on disulfide Zn chelators, their role in intermicrobial competition, and defense mechanisms (based on oxidation back to disulfides).
bioRxiv Microbiology@biorxiv_micrbio29 Nov 2023
Convergent evolution in toxin detection and resistance provides evidence for conserved bacterial-fungal interactions biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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New from PhD candidate @YaegerLuke and friends - a fascinating dive into the connections between #folate and #peptidoglycan pathways in #Pseudomonas that inspired Luke to design a dual-function inhibitor that targets both! @McMasterIIDR @BBS_McMasterU 1/11 biorxiv.org/content/10.1101/…

Metabolic connections between folate and peptidoglycan pathways in Pseudomonas aeruginosa inform...

Peptidoglycan is an important bacterial macromolecule that confers cell shape and structural integrity, and a key antibiotic target. The synthesis and turnover of peptidoglycan are carefully coordi...

biorxiv.org
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Finally 🎉! From @YaegerLuke et al: Central metabolism is a key player in E. coli biofilm stimulation by sub-MIC antibiotics @jpcotelab @eric_brown_bbs @McMasterIIDR 1/n journals.plos.org/plosgeneti… 1/8

Central metabolism is a key player in E. coli biofilm stimulation by sub-MIC antibiotics

Author summary Treatment of bacterial pathogens with subinhibitory concentrations of many different types of antibiotics can have the perverse effect of increasing biofilm formation, making infecti...

journals.plos.org
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Here’s the latest from @YaegerLuke and friends! We’ve been studying this common but poorly understood phenomenon for a while. “A genetic screen identifies a role for oprF in Pseudomonas aeruginosa biofilm stimulation by subinhibitory #antibiotics” biorxiv.org/content/10.1101/… 1/4

A genetic screen identifies a role for oprF in Pseudomonas aeruginosa biofilm stimulation by...

Biofilms are surface-associated communities of bacteria that grow in a self-produced matrix of polysaccharides, proteins, and extracellular DNA (eDNA). Sub-minimal inhibitory concentrations (sub-MIC)...

biorxiv.org
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A feedback control mechanism governs the synthesis of lipid-linked precursors of the bacterial cell wall biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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A new paper from our lab is out today after peer review: science.org/doi/10.1126/scie…

Emergent coexistence in multispecies microbial communities

Analysis of stable synthetic bacterial communities suggests that coexistence is an emergent property of complex communities.

science.org
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