Developmental biologist @COSHeidelberg @WittbrodtLab chalk-talker, CRISPRer, Baseeditor & glycosylation & CDG modeler.
Heidelberg
Joined October 2013
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Want to base edit and no PAM in reach? Just create one! "Inception" is a one-shot event based on a canonical target site to create a de novo PAM and a 2nd editing upstream, relaxing steric dependencies. #CRISPR #baseedit with @kaisa_pakari, @WittbrodtLab
biorxiv.org/content/10.1101/…
Calling all #medaka PI researchers! Join us in Heidelberg, Germany July 22-24, 2025 for our collaborative meeting! Register now meetmedaka.com/registration/ & take advantage of early bird rates until April 15th! 🐟 #MedakaResearch #ModelOrganisms #ScienceConference #FishModels #fish
Thomas Thumberger retweeted
New high-throughput optomotor response (OMR) assay for medaka fish larvae reveals strain-specific visual function differences & training effects. Our open-source setup enables studies on visual acuity, color sensitivity & more #NeuroBiology #FishResearch 🐠journals.plos.org/plosone/ar…
Thomas Thumberger retweeted
That's it: #GfE2024 is over. Thanks everyone for the science, amazing talks, chairing, buzzing poster sessions, short & long discussions, organizers in the back & front - simply thanks for all your contributions! See you all latest at #GfE2026 for updates & new routes of #DevBio
and furthered by comparison with other regenerating and non-regenerating fish like the surface and cave phenotypes of #astyanax. impressive talk at #GfE2024!
Mathilda Mommersteeg (@tmommersteeg) - as fish is not fish, "Identifying the metabolic profile underlying successful fish heart regeneration" is possible through the different #zebrafish strains available. #GfE2024
very nice talk from @Adelmann_L on #platynereis. She makes use of #scTranscriptomics over time during the course of regeneration and finds stem cell hotspots in different clusters 💪 #GfE2024
Leonie Adelmann (@Adelmann_L) is "Dissecting the re-establishment of stem cells for hormone-dependent posterior regeneration in a marine annelid" #platynereis #stemcells #DevBio #GfE2024
If you desperately need to explain the problem of in vitro recombination in #PCR fooling many to believe #CRISPR/#HDR events went smooth, use the remainder space on your poster 😂 read up here for fish elifesciences.org/articles/3… & here for the mouse academic.oup.com/nar/article… #GfE2024
Thomas Thumberger retweeted
Announcing the fantastic keynote speakers for the 11th AQMHD Conference: Leonard Zon, Jochen Wittbrodt, and Duygu Ozpolat. Registration and more information can be found here: aqmhd.xiphophorus.txst.edu/.
Thomas Thumberger retweeted
The member councils of the GfE and DSDB just met to discuss future plans #GfE2024. Common topic: high fluctuation of members. Join societies to support us in supporting events and young scientists - it's your annual fee that allows us to. Become a member: vbio.de/gfe-entwicklungsbiol…
Great talk by Christof about one of the hallmarks of #DevBio. Brings back memories of the good time I spent on the organizer during my PhD dissecting its downstream left-right organizing activity. #xenopus #cilia #LROrganizer
#GfE2024 last talk of the Stem cell session. Jochen talking about our SNP validation screen on heart-beat Gene-by-Environment alleles - CRISPR/BaseEditing SNP-fipping validated by CRISPR & ACEofBASEs editing aceofbases.cos.uni-heidelber…
Jochen Wittbrodt (@WittbrodtLab) "genetics of individuality" population genetics based on our inner fish. How SNPs influence traits like the 🫀 heart beat in a Gene-by-Environment dependency (GxE) #medaka #GWAS
Thomas Thumberger retweeted
Open PhD position in Heidelberg. Please RT!
Do you want to identify the molecular mechanisms regulating genomic and chromosomal mosaicism in the developing brain? We seek a motivated doctoral researcher @COSHeidelberg @UniHeidelberg. Apply by 15 April here euraxess.ec.europa.eu/jobs/8…
CRISPR-Fortschritte - Schnitt für Schnitt zu neuen Möglichkeiten: unser Review über sukzessive, kleine Fortschritte, die gezieltes Genomeditieren mittels CRISPR & BaseEditoren effizienter gemacht haben
link.springer.com/article/10… ✂️ mit @kaisa_pakari & @WittbrodtLab im @Spektrum_BIO
I guess this meeting is focusing on zebrafish fin mutants 😉
Mark your calendars: The next Mid-Atlantic Regional #Zebrafish meeting will be held May 26th at the NIH! See our website for more information: sites.google.com/view/marz20…
In contrast to PAM-less editors, inception uses target-specific NGG-editors. Chaining 2 guides & 2 editors is predictive & efficient. Though simultaneously applied, mode of action is sequential. 2nd edit depends on the 1st - allowing editing 27-36nt upstream of the canonical PAM
published @Dev_journal now: journals.biologists.com/dev/…
ahhh, bench air! Dear #miniprep, how I missed you! You routine chance for self-optimization. You simple process that grants room to think while doing, that makes automated movements happen thanks to imprinted memory in my Medulla spinalis. Even after years of abstinence. Thank U!
Thomas Thumberger retweeted
Verfahren der Genom-Editierung optimiert: Heidelberger Wissenschaftlern gelingt es, die Effizienz von CRISPR/Cas9 sowie verwandten Methoden zu steigern und bislang unerreichbare DNA-Sequenzen zu modifizieren uni-heidelberg.de/de/newsroo…
ALT Nach erfolgreicher oca2-Gen-Editierung spiegelt das Verhältnis von ursprünglich pigmentierten zu unpigmentierten Zellen im Auge von Embryonen des Japanischen Reiskärpflings Medaka die Effizienz der Cas9-Aktivität wider. Dies wurde genutzt, um das CRISPR/Cas9-System zu optimieren. Wenig effektiver oca2-Verlust mit gängigen Cas9-Varianten (oben) und stark gesteigerte Effizienz durch Nutzung der heiCas9 (unten). © Thomas Thumberger (COS)
Just published @Dev_journal, Kaisa (@kaisa_pakari) presents our "inception" approach to target previously inaccessible nucleotides with base-editing at the 1st Heidelberg #CRISPR mini symposium @MKolleg today - link to paper: journals.biologists.com/dev/…
Thomas Thumberger retweeted
Here it is: our #inception paper @Dev_journal „De novo PAM generation to reach initially inaccessible target sites for base editing“ by @kaisa_pakari & @_ThomThum_ We use 2 guides & 2 editors to edit distant nucleotides with NGG base-editors
journals.biologists.com/dev/… #medaka #DNA