Postdoc studying planarian regeneration @ the Stowers Institute in Kansas City. My friends call me Biff.
Kansas City, Missouri
Joined July 2021
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Frederick Mann, PhD retweeted
Our new NAFA protocol robustly preserves delicate wounded tissues while facilitating probe/antibody penetration into internal tissues
The fixation protocol is compatible for WISH on regenerating teleost fins, highlighting its versatility
@ScienceStowers @BMCBiology
bmcbiol.biomedcentral.com/ar…
ALT FISH of zpuf-6 (yellow) and DNA staining with DAPI (white). Maximum intensity projection of confocal images, scale bars: 100 μm
ALT ISH in killifish tail fin yields better signal to noise ratio when fixed with PFA + formic acid. A Morphology of the tail fin in the African killifish N. furzeri. Amputation site is indicated by red dashed line. B Chromogenic in situ hybridization of the injury-responsive gene fstl1. 1 dpa and 3 dpa tail fin tissues fixed with 4% PFA and probed for fstl1.C ISH for fstl1 in tail fins fixed with 4% PFA + formic acid. Brightfield images were taken with a stereomicroscope. Scale bars are 500 μm
Frederick Mann, PhD retweeted
The @MBLScience Embryology Course is underway! 🔬🌊 Institute researchers from the @Planarial Lab, @Vidyanandsasi and @DungVuu are teaching assistants at #Embryo24. There is no better place to spend the summer than our satellite lab in #WoodsHole. #marinebiology
#science
Frederick Mann, PhD retweeted
We just posted a new version of our planarian spatial transcriptomics story! Lots of new analysis & RNAi functional testing. Check it out!
@ScienceStowers @fg_mann @DungVuu @S_Nowotarski @Planaria1 biorxiv.org/content/10.1101/…
Frederick Mann, PhD retweeted
Many many congratulations Dr @VirajDoddihal.. your beginners luck for that use of sword epic cOool 😍🤩 happy happy times Dr. Enjoys enjoys the glory of all ur hard work 🍾🥂😃🤩
Frederick Mann, PhD retweeted
Excited to announce the planarian transgenic workshop at Stowers, Nov. 7-10, organized by @Planaria1 and us, fully funded by NSF EDGE. Come to transfect worms and build your own luminescence microscope to monitor gene expression. Register at stowers.service-now.com/visi…. Please RT.
Frederick Mann, PhD retweeted
How does a small fragment of planarian regenerate an appropriately proportioned complete animal?
As part of my PhD project I attempted to address this question with @Planaria1 @fg_mann , Eric Ross, & Sean McKinney @ScienceStowers @HHMINEWS
Preprint: tinyurl.com/2hvb9j4z
Frederick Mann, PhD retweeted
What a year it has been @ScienceStowers !! This year as a post baccalaureate research scholar @GSSIMR has been nothing but amazing to me. I’ve learned SO much and I can only thank the @Planaria1 lab, @fg_mann for being the greatest mentor I could ever ask for.. 1/2
Frederick Mann, PhD retweeted
Today we celebrate Enya @DewarsEnya who a year ago joined our institute @ScienceStowers & our lab as a postbaccalaureate
Enya will pursue her PhD @DukeU this fall
While we will miss her, we wish her a world of new knowledge & discovery will always come to greet her
Go science!
Frederick Mann, PhD retweeted
Dr. Biff Mann (@fg_mann) from @Planaria1 lab will discuss a genome-wide Smed RNAi library, and Pallob Barai from @BethMDuncan lab will speak about an RNase H-based ribodepletion strategy for transcriptomic profiling.
We look forward to seeing you there!
2/2
Frederick Mann, PhD retweeted
All of us will be presenting this work at this Friday’s #FlatwormFriday! So tune in!
Frederick Mann, PhD retweeted
It was a great experience writing a chapter on diverse types of #regeneration with @Planaria1 @ScienceStowers . Can’t wait to get my hands on this book to read all the other exciting chapters.Thanks for the opportunity @CSHLPress @Ken_Poss_Lab @FoxLabDuke. Loved the cover page!
Hot of the @CSHLPress ! New #regeneration book! Thanks @Ken_Poss_Lab for the opportunity to co-edit. cshlpress.com/default.tpl?ac…
Frederick Mann, PhD retweeted
Congratulations to @planaria1 for his election to #AAASFellow by @AAAS. Fellows are selected for their achievements & extraordinary efforts on behalf of the advancement of science.
Read more about the awardees here: aaas.org/news/aaas-honors-ou…
Frederick Mann, PhD retweeted
Sonpho et al. provide an experimental approach for identifying both known and novel ECM components involved in regeneration — @Planaria1 @ScienceStowers @MahidolU #ECM #TeamMassSpec
mcponline.org/article/S1535-…
Frederick Mann, PhD retweeted
From the laboratory of @Planaria1 @ScienceStowers, our paper identifying unsuspected roles for Hox genes in adult tissues is now out in @NatureComms. Here is a summary thread of the story. @HHMINEWS @NIGMS. #hoxgenes #invertebrates
1/16
nature.com/articles/s41467-0…
Frederick Mann, PhD retweeted
Sharing the latest preprint from @Planaria1 lab describing a protocol for WISH and immunostaining while preserving delicate tissues. Carlos is the superstar behind this protocol and was such a fun collaboration with @fg_mann
@ScienceStowers @GSSIMR
tinyurl.com/7xwssp3n
Frederick Mann, PhD retweeted
So excited to see this protocol out so folks can use it! Great job Carlos, @VirajDoddihal and @fg_mann!
Sharing the latest preprint from @Planaria1 lab describing a protocol for WISH and immunostaining while preserving delicate tissues. Carlos is the superstar behind this protocol and was such a fun collaboration with @fg_mann
@ScienceStowers @GSSIMR
tinyurl.com/7xwssp3n
Frederick Mann, PhD retweeted
Got a newer research organism & want to build tools the established ones have?
@Sofia_Robb, @eldavies03, @Planaria1 & I are excited to share the Planarian Anatomy Ontology(PLANA) that defines & organizes planarian anatomy in @Dev_journal.
bit.ly/3sEK09D
(1/n)
Frederick Mann, PhD retweeted
Do you have a research organism with few markers but for which you would like to know cell population composition and cellular phenotypes?
Image3C may be of help
Paper now out @eLife
doi.org/10.7554/eLife.65372 @eLife
@ScienceStowers
@accorsi_alice @robertpeuss @NicolasRohner
Frederick Mann, PhD retweeted
When a bilaterally symmetric organism studies another bilaterally symmetric organism and one of them uses the other to make radially symmetric compositions…
Julianna Haug, research associate in the Sánchez Alvarado Lab, studies the how stem cell metabolism influences cell fate in Planaria flat worms. Here RNA-ISH marks sub-epidermal glands (red), gut (dark-orange), basal lamina (orange), and DNA (yellow). @Planaria1 @JuliannaHaug
Frederick Mann, PhD retweeted
Julianna Haug, research associate in the Sánchez Alvarado Lab, studies the how stem cell metabolism influences cell fate in Planaria flat worms. Here RNA-ISH marks sub-epidermal glands (red), gut (dark-orange), basal lamina (orange), and DNA (yellow). @Planaria1 @JuliannaHaug