*This Twitter account is not monitored any longer. Please join me on LinkedIn for continued research updates.* --- Florian, PI in Advanced Imaging
The Arctic University (Norway)
Joined October 2016
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Florian Ströhl retweeted
This is _clearly_ one of the best ideas in imaging of the decade.
I strongly encourage people to play around with this if they have a few spare hours — watching a *dye* clear a tissue in front of your eyes for the first time is an amazing experience.
Long awaited (thanks @JamesDManton for posting the patent application!) and now out in @ScienceMagazine: Using absorption to look deeper by @HongNeuroTech - they achieved approx. 3 mm depth using the food dye tartrazine: #Microscopy
science.org/doi/10.1126/scie…
Florian Ströhl retweeted
We present:
'Multispectral live-cell imaging with uncompromised spatiotemporal resolution'.
biorxiv.org/content/10.1101/…
1/16
Recording many image planes simultaneously is a great time saver for 3D imaging. A wonderful tool for such multifocus microscopy is @FourierPower's Multifocus Grating: Here is a short video on how they work and how to make them (without a cleanroom). youtu.be/4K_KcILtAMo?si=T48w…
Snouty-style OPMs offer volumetric imaging with high spatial- and temporal resolution, but the setups can suffer from polarization-dependent light reflections which reduce the effective aperture and transmission in the remote image space. 1/n
If you want to figure out the theoretical limits of your own LSM system, we also provide a software tool that can simulate any light-sheet microscope. The tool has a GUI and is straightforward to use. 3/n doi.org/10.18710/YAYNNL
You can read the full paper (open access) at nature.com/articles/s41598-0…. Finally, thanks a lot to Jon-Richard Sommernes and @amsikking - it was a pleasure working with you on this project! 4/n
Florian Ströhl retweeted
How to draw optical layout / schematics?
First, start with vector graphics tool. Inkscape is free and good.
Secondly, there are libraries of nicely drawn basic optical elements such as gwoptics.org/ComponentLibrar… @gwoptics & Alexander Franzen
1/N
What a start to 2024. Nobel Prize winner @Eric_Betzig and his team not only built a SOLIS system. No, they built 4!
doi.org/10.1101/2023.12.21.5…
Here is the original SOLIS paper that explains how the method can capture entire optically sectioned volumes at the camera framerate:
doi.org/10.1364/OPTICA.46858…
Florian Ströhl retweeted
🤫 #chatGPT integration will be coming to @FijiSc's Script Editor #SneakPreview 🥳
Thanks for the support @ctrueden ! 🙌
github.com/scijava/script-ed…
Florian Ströhl retweeted
The slight disappointment with being scooped is more than compensated by the very nice results and demonstrations of reduced photobleaching/toxicity with NIR co-illumination in this paper by Ludvikova et al: doi.org/10.1038/s41587-023-0…
Here's how we did it: nature.com/articles/s41377-0…
.. of course with complementary YouTube video: youtu.be/2_01bQVeJn4
nature.com/articles/s41587-0…
Fantastic work by Hari Shroff's group on 4-beam SIM.
An impressive experimental demonstration of 4-beam 3D-SIM for enhanced axial resolution, by Hari Shroff's lab: biorxiv.org/content/10.1101/…
Remember Avatar, the first 3D movie? Yeah, that wasn't 3D. Actual 3D needs more than polarising glasses.
Let's develop a real 3D imaging system together, shall we? youtu.be/JAnzREt6IZE
Details in our newly published Optica paper 🥳: doi.org/10.1364/OPTICA.46858…
It's written in the stars: there's a new fluorescence microscopy paper coming from the Arctic University :)
James nails it: the best reason to enjoy the good view from your lab is "alignment purposes"
Florian Ströhl retweeted
1/? I've had a few people ask me why I prefer @Thorlabs posts over their cages. The argument for cages is self-defeating: supposedly they provide pre-aligned centration, but mechanical tolerances =/= optical tolerances. You end up having to put everything on kinematic mounts...
