genefish

Oysters exposed to 35°C heat for 24 hours showed significantly lower glycogen. Recovery may partially restore levels, but a history of heat stress didn’t significantly affect glycogen after a subsequent heat exposure. #Oysters #ClimateStress ift.tt/InAGiVT

A water-chemistry trial tank continues to show elevated ammonia and nitrite, unlike the main holding tanks. A study in Pacific oysters links sustained ammonia exposure to immune-cell death—making these readings a potential stress signal, not just a numbe… ift.tt/tvKgTFc

Tank-room trial: tanks held 30 ppt salinity, but water chemistry varied. The oyster trial water showed the highest ammonia (0.25+), nitrite (1.5) and nitrate (60), with pH 7.4. ift.tt/yYF3jRb

AI measured oyster dimensions from photos, closely matching manual results—with a few outliers needing review. A recent study also shows deep learning can accurately assess oyster shells from images, supporting AI’s promise in oyster research. ift.tt/OKQSP2l

Tested my AI tool on oyster images with many specimens and clean backgrounds. Measurements closely matched manual results, with a few errors corrected by hand. Next: compile a comparison dataset to help future lab members decide when to use it. ift.tt/Nf2hz1B

This week’s oyster research showed a mixed picture: El Niño-like conditions drove high mortality, while 36°C heat caused complete mortality by 96 hours. Yet freshwater and thermal hardening did not significantly improve survival in the Goose Point assay.… ift.tt/4uAFx68

Oyster findings connect: collapsed alkalinity coincided with rising ammonia, while resazurin assays showed higher metabolic activity at 30°C than 11°C. Sustained extreme heat killed all remaining oysters—underscoring how water chemistry and temperature s… ift.tt/rcGn8Fk

Ran a GlycogenGlo assay to measure glycogen in samples from a 35°C repeat-stress experiment. Data analysis coming soon! #Glycogen #Research ift.tt/UOzesQb

🧪 Tank room water chemistry trial: salinity held at 30 ppt across tanks, but alkalinity, nitrite and nitrate varied. Oyster trial water measured 0.25+ ammonia, 1.5 nitrite and 50+ nitrate—key parameters to monitor. #Aquaculture #WaterQuality ift.tt/RSO9VxQ

🧪 Research priorities for 10/24–11/07: discuss oyster results and cross-tolerance assays, improve starfish transcriptomics plots with ggplot2, learn DESeq2, and coordinate meetings with Dr. Roberts and Grace. #Research #Transcriptomics ift.tt/snrY0v1

Processed M. gigas resazurin assay data with Sam White’s R script. Early results suggest oysters held at 30°C had the highest metabolic activity, while those at 11°C had the lowest. Next: investigate anomalous readings and validate the interpretation. ift.tt/c8oRkCU

The oyster heat stress experiment has concluded: by 96 hours, all three remaining oysters had died—one each from the low, control, and high groups. ift.tt/84weOJm

Oyster water quality is more than a nuisance: ammonia can harm immune cells, and alkalinity may affect ammonia excretion. A trial tank’s zero alkalinity and elevated ammonia, nitrite and nitrate could compound physiological stress. #Oysters #Aquaculture ift.tt/1eqU7lA

🦪 Water chemistry varied across the tanks: salinity held at 30 ppt, but alkalinity ranged from 0–80. The oyster trial water showed 0.25+ ammonia, 1.5 nitrite, and 50+ nitrate—key conditions to monitor. #Oysters #WaterChemistry ift.tt/FhAu4oE

Oysters completed 24-hour heat (35°C) and control (15°C) exposures. A subset was sampled for qPCR; the rest will recover for a week before reciprocal exposure and sampling. #Oysters #HeatStress #qPCR ift.tt/wSfsbnu

At 36°C, 13 of 17 oysters died within ~72 hours, across all treatment groups. Research suggests heat exposure duration and individual factors—such as shell condition and prior stress—may shape survival. #Oysters #HeatStress ift.tt/M48rnp9

After ~72 hours at 36°C, the oyster heat-stress experiment recorded 13 deaths, with only 4 oysters still alive—a stark sign of the toll prolonged heat can take. ift.tt/3uDe1x0

Oyster stress studies: 24-hour exposure at 35°C is underway to improve RNA/qPCR samples. A 37°C priming test sets up next week’s 44°C lethal challenge—but keeping water temperature consistent remains a hurdle (36–39°C). ift.tt/XEjL1Z4

Heat stress at 36°C led to 26 oyster mortalities. The 30 survivors from the shelf system were measured and tested with a resazurin assay, monitored every 30 minutes for 3 hours. ift.tt/Y2TNmvU

Oyster hardening assays coincided with degraded tank conditions: low alkalinity and elevated ammonia/nitrite. A field study suggests nitrite may be a secondary survival driver, but alkalinity and ammonia still need investigation. #OysterFarming ift.tt/ZeLYRD9