LenaKSchroeder (@lenakumba) profile picture

LenaKSchroeder

@lenakumba

Associate Director of Cellular Imaging core, Fred Hutch, super-resolution microscopy enthusiast #STED #4PiSMS, cell biologist #ER All opinions are my own

 Seattle, WA
Joined November 2008
  • Tweets48
  • Following144
  • Followers178
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Photo by LenaKSchroeder (@lenakumba)Photo by LenaKSchroeder (@lenakumba)Photo by LenaKSchroeder (@lenakumba)
Making rounds at the poster session #ascb #cellbio2023
Image from LenaKSchroeder (@lenakumba)
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Green Green and Blueberry spin the wheel #ascb #cellbio2023
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Green Green the sea turtle and Blueberry the husky attending their first #ASCB #cellbio2023
Image from LenaKSchroeder (@lenakumba)
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.@freethepipette, @fran_bottanelli and colleagues combine gene editing and STED super-resolution #microscopy to reveal the nanoscale localization of ARF #GTPases in living cells bit.ly/3HhBHcp
Image from Journal of Cell Biology (@JCellBiol)
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Congrats Sarah!
JakobsLab (@JakobsLab) profile pictureJakobsLab@JakobsLab6 Feb 2023
They grow so fast! Our PhD student @AminoSarah16 is now a full PhD! 👨‍🎓Congratulations on this great doctoral defense! 🥳🍾🎉
Image from JakobsLab (@JakobsLab)
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ICYMI at #cellbio2022 Shirin Bahmanyar named 2022 WICB Junior Award for Excellence in Research ascb.org/member-news/shirin-…
Image from American Society for Cell Biology (@ASCBiology)
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Hey #science twitter! We have update our preprint to showcase the endogenous tagging with TurboID pipeline with datasets with the native proximity proteome of all adaptor protein complexes! #membranes #MS #CRISPR biorxiv.org/content/10.1101/…
When less is more – Endogenous tagging with TurboID as a tool to study the native interactome of...

When less is more – Endogenous tagging with TurboID as a tool to study the native interactome of...

In recent years, proximity labelling has established itself as an unbiased and powerful approach to map the interactome of specific proteins. Generally, protein fusions with labelling enzymes are...

biorxiv.org
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Out today from @bewersdorflab! Two-color fluorogenic DNA-PAINT introduces self-quenching, kinetics-optimized probe designs. This approach improves imaging speed 26-fold and eliminates the need for optical sectioning. nature.com/articles/s41592-0…
Image from Nature Methods (@naturemethods)
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Small but mighty! This is a video of a killer T cell of the immune system destroying a monstrous ovarian cancer cell. I recently captured this data on a spinning disc confocal microscope.
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“Cryo-ExM” or “how to see (almost) everything you ever dreamt of” Want to know more ?!? Check our story out with @CentrioleLab @nikolai_klena in @naturemethods: nature.com/articles/s41592-0… which combines cryo-fixation/Freeze substitution with #expansion #microscopy (#UExM).
Image from Marine Laporte (@MarineLaporte8)
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LenaKSchroeder retweeted
Dr. Anna Mammel (@DrMammel) profile picture
Dr. Anna Mammel@DrMammel14 May 2021
I just posted my first research article as a #TeamHatchLab post doc to @BioRxiv and I’m so excited to share it with you in this thread! “Chromosome length and gene density contribute to micronuclear membrane stability” 🧵 biorxiv.org/content/10.1101/… 1/13
Chromosome length and gene density contribute to micronuclear membrane stability

Chromosome length and gene density contribute to micronuclear membrane stability

Micronuclei are derived from missegregated chromosomes and frequently lose membrane integrity, leading to DNA damage, innate immune activation, and metastatic signaling. Here we demonstrate that two...

biorxiv.org
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2-photon excitation + adaptive optics + red dyes + #STED = 3D super-resolution imaging ~100 microns deep in the brain of a living 🐭! Our paper is now out in #OSA_Optica. Congrats Mary Grace Velasco et al.! 🙌🔬doi.org/10.1364/OPTICA.41684… @OSAPublishing @YaleCellBio @YaleBme
Image from Bewersdorf Lab (@bewersdorflab)
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Remarkable images of mitochondria and Golgi from @OnsMSaad1! I love the flexibility of combining protein specific antibody labeling, organelle specific stains, and primary amine labeling to see exquisite details of the cell.
Bewersdorf Lab (@bewersdorflab) profile pictureBewersdorf Lab@bewersdorflab15 Mar 2020
Our most recent super-resolution development is now on bioRxiv: pan-ExM generates images which look remarkably similar to FIB-SEM but require just a normal confocal microscope. (1/2) #panExM @OnsMSaad1 biorxiv.org/content/10.1101/…
Light microscopy of proteins in their ultrastructural context

Light microscopy of proteins in their ultrastructural context

Resolving the distribution of specific proteins at the nanoscale in the structural context of the cell is a major challenge in fluorescence microscopy. Here we present a new concept that decrowds the...

biorxiv.org
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Replying to @YongdengZhang
@YongdengZhang and @lenakumba's 'salvaged fluorescence' multicolor super-resolution paper in collaboration with @DavidBaddeley5, Jim Rothman and @PDClab of @YaleCellBio, @rhodamine110, @JonasRies and colleagues is now out in Nature Methods. Great start to 2020! 🎉🥳🍾
Nature Methods (@naturemethods) profile pictureNature Methods@naturemethods6 Jan 2020
4Pi single-molecule switching microscopy combined with ‘salvaged fluorescence’ enables improved ratiometric imaging that bypasses chromatic aberrations and allows for multicolor whole-cell imaging with sub-10-nm localization precision. nature.com/articles/s41592-0…
Image from Nature Methods (@naturemethods)
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The Summer issue of Yale Medicine Magazine is out! Focused on technological innovations at YSM, it features the creativity of engineers, scientists, and doctors like Jeorg Bewersdorf, PhD, who are working together to push the limits of what's possible. medicine.yale.edu/news/yale-…
Image from Yale School of Medicine (@YaleMed)
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from @PDClab, use of DNA-origami nanostructures supports a model where lipids are transferred between ER and plasma membrane through a shuttle mechanism mediated by the synaptotagmin E-Syt1 go.nature.com/2YTUTol (free-to-read at rdcu.be/bKZq2)
A programmable DNA-origami platform for studying lipid transfer between bilayers

A programmable DNA-origami platform for studying lipid transfer between bilayers

Nature Chemical Biology - Use of DNA-origami nanostructures to study lipid transfer between closely apposed membrane bilayers supports a model where phospholipids are transferred by extended...

nature.com
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LenaKSchroeder retweeted
Bahmanyar lab (@Bahmanyarlab) profile picture
Bahmanyar lab@Bahmanyarlab20 Jul 2019
I love the #howcellbiologistswork blog series by #COMPASS @ASCB - thank you to @KiraTheExplora for taking the time to write these relatable pieces to showcase the diversity of paths and approaches to a career in science.
American Society for Cell Biology (@ASCBiology) profile pictureAmerican Society for Cell Biology@ASCBiology19 Jul 2019
Shirin Bahmanyar @Bahmanyarlab emphasizes promoting diversity in stem in this month’s edition of #howcellbiologistswork, a #COMPASS blog series. ow.ly/VzEV50v66Tt
Image from American Society for Cell Biology (@ASCBiology)
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Know somebody interested in #superresolution #STED and #membranetrafficking? We have a fully funded PhD position to work on a project in SFB958 "Scaffolding of Membranes - Molecular Mechanisms and Cellular Functions"! As a + we’ll soon have our own STED scope! RT are appreciated
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LenaKSchroeder retweeted
Till Stephan (@stephan_till) profile picture
Till Stephan@stephan_till18 May 2019
I still remember how fascinated I was about the complex cristae architecture when we took the first images. #livecell #sted #mitochondria – at Göttingen, Germany
Image from Till Stephan (@stephan_till)
bioRxiv Cell Biology (@biorxiv_cellbio) profile picturebioRxiv Cell Biology@biorxiv_cellbio17 May 2019
Live-cell STED microscopy of mitochondrial cristae biorxiv.org/cgi/content/shor… #biorxiv_cellbio
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LenaKSchroeder retweeted
Felipe Opazo (@FelipeOpaz_o) profile picture
Felipe Opazo@FelipeOpaz_o18 May 2019
Finally our preprint describing a set of nanobodies binding with different properties to a single epitope tag (ALFA system). A great tool developed by @_NanoTag_. biorxiv.org/cgi/content/shor…. #bioRxiv.
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