Associate Professor at Shenzhen University Medical School Bacterial pathogenesis (S. pneumoniae, K. pneumoniae), Genetic editing of non-model bacteria

Shenzhen, China
Joined September 2015
xue liu retweeted
The older I get the more I realise, maturity is simply becoming more honest with yourself. If you want to rest, you rest. If you want to work, you work. You don’t say yes, when you want to say no. You don’t do things that drain you. You stop hanging out with people who you have to perform for. You fall in love with the simple, ordinary things that your heart actually craves for. Simplicity and peace become your highest priority. That’s all maturity is about.
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Thrilled to be back in Europe after two years. Especially excited to attend the EMBL Symposium again, first time since 2019! Looking forward to great science and inspiring discussions. #EMBLSymposium #microbiology
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Great job opportunity at The Center for Integrative Genomics, Lausanne University, Switzerland unil.ch/cig/en/home.html For a tenure-track Assistant Professor position. More details for how to apply are here nature.com/naturecareers/job…
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Short‐term #probiotic supplementation affects the diversity, genetics, growth, and interactions of the native gut #microbiome onlinelibrary.wiley.com/doi/… #guthealth @wileymicrobio @WileyHealth @WileyBiomedical
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Antibiotics that Kill Gram-negative Bacteria by Restructuring the Outer Membrane Protein BamA biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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The @EMBLEvents 2025 Courses and Conferences poster has had an update! If you're especially interested in training in #datascience #AIinScience #omics and #bioinformatics, take a look at the EMBL-EBI courses here: ebi.ac.uk/training/live-even…
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Tax1bp1 enhances bacterial virulence and promotes inflammatory responses during Mycobacterium tuberculosis infection of alveolar ... biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response against a pathogen. Could lead to topical vaccines that are applied in a cream. @DjenetBousbaine led the charge... @Nature 1/55
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Hello, I am looking for a #Postdoctoral researcher with experience in bacterial genetics and antimicrobial resistance. Please send me a CV and a brief summary of your lab experience! Thank you!
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The good idea comes when one person feels comfortable enough to say something that might make them look stupid and their friend is open enough to recognize its potential.
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Genome-wide CRISPRi-seq identified ferredoxin-NADP reductase FprB as a synergistic target for gallium therapy in Pseudomonas aeruginosa biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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xitter.cf/biorxiv_micrbio/status… This collaboration work started 3 years ago and was mainly carried out by a master student Ling Liu, who never knew how to culture bacteria at that time.
Equip Fusobacterium nucleatum genetic tool kits with compatible shuttle vectors and engineered intermediatory E. coli strains for ... biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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Hope our work can contribute a bit to the research related to F. nucleatum.
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All the tools developed in this study was shared via the WeKwikgene (wekwikgene.wllsb.edu.cn/).
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Additionally, we evaluate promoters with variable strengths with a luciferase-based reporter system in F. nucleatum, providing valuable insights for future gene editing studies in bacterium and contributing to our understanding of its pathogenesis.
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By exploring the native plasmids from F. nucleatum, we identified new backbones for construction of shuttle vectors and established a dual-plasmid system for the first time, offering new avenues for genetic manipulation in this bacterium.
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The conjugation-based DNA delivery to F. nucleatum was also established, and this strategy can bypass the requirement of MTase methylations.
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We employed Pacbio sequencing and identified the active RM systems in F. nucleatum. Engineered intermediatory E coli strains to expression the Methyltransferases. Improved the transformation efficiency 1000 fold, reaching 5000 CFU per ug plasmids.
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