Associate Professor for Molecular Evolution @univgroningen ; passionate scientist and insomniac. News, views & random things
Groningen, Nederland
Joined June 2014
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🥳🥳🥳 Our study on engineering enzymes to break C–F bonds is now out in @angew_chem! Congrats to the brilliant & dedicated Suzanne Jansen for taking this first step toward the future bioremediation of #PFAS & other contaminating organofluorides!
onlinelibrary.wiley.com/doi/…
🚨New preprint! What a difference one C-F bond can make!🚨
Fluoroacetate dehalogenases (FAcDs) are remarkably good at breaking C-F bonds. But add just ONE more C-F bond to the substrate and suddenly the enzyme is inhibited.
So what’s going on? (1/4)
biorxiv.org/content/10.64898…
Substituting this histidine with a non-canonical histidine analog or Gln can overcome the inhibition! A beautiful example of modern mechanistic enzymology and an important step toward our longer-term goal of making FAcDs fit for PFAS degradation. 🌍
One C-F bond at a time! (3/4)
Huge credit to @suzanne__jansen, who led this work. But without Luis Guerra & Manuel Müller from @lab_mueller, as well as Cristina Duran & @silviaosu, we could have never provided experimental and computational evidence for isoaspartate formation! 🤝 (4/4)
🧵 New review out in Chemistry – A European Journal!
Hybrid macrocyclic peptides: combining biological selection with synthetic chemistry to discover next-generation therapeutics.
Why are hybrid macrocyclic peptides so exciting? 👇
(1/5)
chemistry-europe.onlinelibra…
📢 📢 📢 !!!Come and join us!! 📢 📢 📢
We are looking for a Postdoctoral Researcher with in-depth experience in next- and third-generation sequencing to help us understand the fitness landscapes of complex biomolecules! Please spread the word :)
werkenbij.rug.nl/vacature/po…
With #PFAS and other organofluorides wreaking havoc, we set out to engineer natural defluorinases for man-made organofluorides. All credit goes to the amazing @suzanne__jansen, who has outdone herself in this project! (1/5)
biorxiv.org/content/10.1101/…
Forcing populations of bacteria expressing tens of thousands of distinct FAcD-variants to utilize various organofluorides as sole carbon source, we elicited a panel of FAcD variants with improved activities and drastically altered substrate profiles. (4/5)
narf42 retweeted
So glad to see this out! By giving a strong fluorescence signal to read out NAD(P)H concentration, SAFRAN will greatly extend the range of reactivities comfortably assayable in droplets. This will make droplet microfluidics a much more versatile tool for biocatalyst engineering!
Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H: a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets | JACS @hollfelderlab
@Cam_Biochem @Cambridge_Uni pubs.acs.org/doi/10.1021/jac…
Two weeks ago, we had a great group outing, including a city tour through Utrecht, a Mario Kart Wii tournament, and some Rock Band Wii fun! I am truly privileged to work with such a group of talented, dedicated, and smart individuals.
narf42 retweeted
🚨Open PhD positions @TUeindhoven & @univgroningen!
Are you intrigued by the potential of ML+directed evolution in drug discovery (@fra_grisoni), peptide catalysis (@robpollice) or biocatalysis (@narf42)?
Do you like interdisciplinary, cutting-edge research?
Then apply!👇
📢📢📢The ML-GUIDE team (@fra_grisoni, @robpollice and myself) are looking for 3 PhD students, passionate about combining the directed evolution of diverse biomolecules with deep learning approaches to develop better (bio)catalysts and drugs!
Pls retweet
tinyurl.com/3jz97ter
📢📢📢The ML-GUIDE team (@fra_grisoni, @robpollice and myself) are looking for 3 PhD students, passionate about combining the directed evolution of diverse biomolecules with deep learning approaches to develop better (bio)catalysts and drugs!
Pls retweet
tinyurl.com/3jz97ter