Genome Editing, Genome Engineeirng
Joined September 2014
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Our latest @NatureProtocols innovative work to synthesize loooong ssDNA HDR templates. Easi #CRISPR HDR process outpaces NHEJ efficiently inserts foreign DNA. Proud of this work in collaboration with Masato Ohtshuka @biorxivpreprint ---> @biorxiv_pubd nature.com/articles/nprot.20…
Rolen Quadros retweeted
Thread. In a life of 90 years so far, Professor C. N. R. Rao has been in the public academic space for about 60 years or more. It is easy—and incorrect— to pick instances (each of us has no shortage) of real and imagined flaws and make that the narrative.
Rolen Quadros retweeted
The star of the night - Mohd. Shami bags the Player of the Match Award for his incredible seven-wicket haul 🫡
Scorecard ▶️ bit.ly/INDvNZ-SF1-CWC23
#TeamIndia | #CWC23 | #MenInBlue | #INDvNZ
Rolen Quadros retweeted
Congratulations @imVkohli 🎊 Legendary
𝗙𝗜𝗙𝗧𝗬 𝗢𝗗𝗜 𝗛𝗨𝗡𝗗𝗥𝗘𝗗𝗦! 💯
A round of applause for the run-machine: VIRAT KOHLI 👏👏
#TeamIndia | #CWC23 | #MenInBlue | #INDvNZ
Rolen Quadros retweeted
Today’s Semi Final has been even more special thanks to stellar individual performances too.
The bowling by @MdShami11 in this game and also through the World Cup will be cherished by cricket lovers for generations to come.
Well played Shami!
Rolen Quadros retweeted
GONAD (Genome editing via Oviductal Nucleic Acids Delivery) is a powerful technology developed by @Masato_Ohtsuka.
Many students ask us for a training opportunity.
Here is a workshop at @VAInstitute
vai.org/event/gonad/?tabUrl=…
Limited spots!
Secure your's before they are gone!
Rolen Quadros retweeted
In Barbados & in the company of greatness! 🫡 🫡
#TeamIndia meet one of the greatest of the game - Sir Garfield Sobers 🙌 🙌
#WIvIND
Rolen Quadros retweeted
Three of the more costly and frequently used reagents in molecular biology are rolling circle amplification (RCA) mix, chemically competent MachOne (or other E. coli) cells, and Gibson assembly mix. Postdoc Julian Willis—who is starting his own lab in the UK soon—developed homemade recipes for each that reduce costs ~3x (or more at large-scale). Links to each recipe:
RCA mix:
docs.google.com/spreadsheets…
Chemically competent MachOne cells:
docs.google.com/spreadsheets…
Gibson assembly mix:
docs.google.com/spreadsheets…
Rolen Quadros retweeted
n/n We are excited to welcome Prof. LS Shashidhara as our new director!
An accomplished developmental biologist with tremendous experience in institutional building, Shashi cares about science, outreach and policy in India & internationally. We look forward to working with him!
Rolen Quadros retweeted
In the @bioRxiv paper we demonstrate SEND system using GEMs.
We used Cas9mRNA as cargo & checked for gene editing in mouse tissues rb.gy/1mlwqk
For this, we needed a mouse model that turns on fluorescent color if gene editing occurred, also known as a reporter mouse.
Rolen Quadros retweeted
Student: Heard jumping mRNAs?
Postdoc: U mean jumping genes/transposons?
S: No, jumping mRNAs
P: Where did U see?
S: New preprint shows it in🐭rb.gy/1mlwqk
P:😮
~hour later
S: Also see🧵What do you think?
P👌
S😕
P: BTW preprint is great for a journal club. Thx
S🤔
Rolen Quadros retweeted
Delivering mRNAs to mouse tissues using the SEND system biorxiv.org/cgi/content/shor… #biorxiv_genomic
Latest preprint @biorxivpreprint from our lab @CRISPRGuru and @Masato_Ohtsuka Delivering mRNAs to mouse tissue using SEND system #CRISPR #SEND #GENEEDITING biorxiv.org/content/10.1101/…
Rolen Quadros retweeted
Vascular smooth muscle cell-restricted Cre driver just published in Nature Cardiovasc Res circumvents previously lethal visceral myopathies. nature.com/articles/s44161-0…