stonechenlab (@stonechenlab) profile picture

stonechenlab

@stonechenlab

the WRC guy

Joined July 2022
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Excited to share the cryo-EM structure of the Retriever-SNX17 complex. We also identify additional ligands that bind to the same pocket in Retriever. Great collaboration with the Chen lab, UTSW SBL and the Turer lab. biorxiv.org/content/10.1101/…
Structural basis for Retriever-SNX17 assembly and endosomal sorting

Structural basis for Retriever-SNX17 assembly and endosomal sorting

During endosomal recycling, Sorting Nexin 17 (SNX17) facilitates the transport of numerous membrane cargo proteins by tethering them to the Retriever complex. Despite its importance, the mechanisms...

biorxiv.org
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Very happy with the collaboration! Appreciate everyone's contributions!
ezra burstein (@ezraburstein) profile pictureezra burstein@ezraburstein7 Jun 2023
I am delighted to share our findings describing the structural organization of Retriever - CCC: biorxiv.org/content/10.1101/… Congrats also to @PeterJCullen1 @brettcollins100 and Markku Varjosalo and teams who also tackled this question.
Structural Organization of the Retriever-CCC Endosomal Recycling Complex

Structural Organization of the Retriever-CCC Endosomal Recycling Complex

The recycling of membrane proteins from endosomes to the cell surface is vital for cell signaling and survival. Retriever, a trimeric complex of VPS35L, VPS26C and VPS29, together with the CCC...

biorxiv.org
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Very exciting! Wonderful collaborations!
Klemens Rottner (@RottnerKlemens) profile pictureKlemens Rottner@RottnerKlemens14 Dec 2022
Very proud to have been able to be part of this: True multi-disciplinary effort to characterise the physical interaction of Arf1 with WRC… Arf GTPase activates the WAVE regulatory complex through a distinct binding site science.org/doi/10.1126/scia…
Arf GTPase activates the WAVE regulatory complex through a distinct binding site

Arf GTPase activates the WAVE regulatory complex through a distinct binding site

The Arf-family GTPases can switch on a central actin regulator named the WAVE Complex to promote actin polymerization.

science.org
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stonechenlab retweeted
Bogdan Lab (@bogdan_lab) profile picture
Bogdan Lab@bogdan_lab10 Oct 2022
PhD and postdoc positions in immune cell response and wound closure dynamics available - Bogdan lab at the Philipps-University of Marburg. If you are interested in, please apply via [email protected]. PIs please retweet!
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Structural basis for directional chitin biosynthesis nature.com/articles/s41586-0…
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Lamellipodia – branching out without WAVE journals.biologists.com/jcs/…
Lamellipodia – branching out without WAVE

Lamellipodia – branching out without WAVE

The formation of lamellipodia requires the branching of actin filaments; this typically involves Rac GTPases and the WAVE regulatory complex (WRC), which connects Rac activation to actin branching...

journals.biologists.com
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A great collaborative effort with @stonechenlab and @RottnerKlemens group! Congratulations to @bojian_ding for all the structural work and #cryoem and all the other authors.
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Check out our recent manuscript in @NatureComms nature.com/articles/s41467-0… where we show how Rac1 GTPase activates WRC complex to promote actin polymerization by Arp2/3 complex.
Structures reveal a key mechanism of WAVE regulatory complex activation by Rac1 GTPase

Structures reveal a key mechanism of WAVE regulatory complex activation by Rac1 GTPase

Nature Communications - Rho-family GTPase Rac1 activates the WAVE complex (WRC) to promote Arp2/3-mediated actin assembly in various processes. Here, the authors determined cryo-EM structures of...

nature.com
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Our Rac1-WRC paper is published in Nature Communications today rdcu.be/cVI70, showing how Rac1 GTPase activates the WAVE complex to promote actin polymerization. Thanks a million to all co-authors for solving this important mechanism!!
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Yesterday, undergrad scholars from the Biochemistry Undergraduate Research Program presented the fruit of their summer research experiences in the form of 3-min talks. Congratulations Braden, Charlie, Cheng, Ciaran, Grant, Lauren, Michelle, Prescott, and Zheng (Sam)!
The 2022 BSRP scholars after a successful Showcase of their 3-min thesis talks.

ALT The 2022 BSRP scholars after a successful Showcase of their 3-min thesis talks.

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stonechenlab retweeted
Carter Lab (@Carter_Lab) profile picture
Carter Lab@Carter_Lab7 Sep 2022
Our structure of dynein-dynactin on microtubules is now out in @Nature! Thanks to @janetiwasa for this beautiful movie showing how cargos might use pairs of adaptors to recruit their motors. nature.com/articles/s41586-0…
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Last week, we welcomed our new first-year class of Biochemistry PhD students! Welcome to @bbmbisu!
Image from BBMB at Iowa State (@bbmbisu)
Image from BBMB at Iowa State (@bbmbisu)
Image from BBMB at Iowa State (@bbmbisu)
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first tweet?
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stonechenlab retweeted
Dipa Sashital (@dsashital) profile picture
Dipa Sashital@dsashital2 Aug 2022
Come join our growing #cryoEM community! Assistant professor position in the @bbmbisu department at Iowa State University. Our @CryoEM_ISU facility is eager to produce more beautiful structures like the one below! Apply here: isu.wd1.myworkdayjobs.com/en…
Cryo-EM reconstruction of the Cas4-Cas1-Cas2 complex with regions of density corresponding to each subunit colored differently.

ALT Cryo-EM reconstruction of the Cas4-Cas1-Cas2 complex with regions of density corresponding to each subunit colored differently.

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