Dean Clift (@DeanClift1) header
Dean Clift (@DeanClift1) profile picture

Dean Clift

@DeanClift1

@MRC_LMB

 Cambridge, England
Joined August 2011
  • Tweets25
  • Following178
  • Followers72
Latest #Biorxiv paper from our lab looks at how Trim7 interacts with its substrates to explain the diverse roles described for this E3 ligase. Following is a short thread summarising the findings (1) biorxiv.org/content/10.1101/…… teapaw.org/JamesLab9/status/15446…
Image from Leo James' Lab (@JamesLab9)
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Fresh preprint from the lab on TRIM21 mechanism. More info in @LeoKiss5 tweetorial here:
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Linking optogenetic clustering to degradation and what it tells us about TRIM21 activation. This and much more in this work by @Deanclift1 Leo James @w_mcewan and many collaborators, including Filipa Santos and @MarianaOsswald @i3S_UPorto
Dean Clift (@DeanClift1) profile pictureDean Clift@DeanClift125 Feb 2021
In @NatureSMB we show how #TrimAway technology works! #TRIM21 molecules must cluster at the target to degrade; #SocialDistancing not good! Embracing this clustering mechanism allows selective degradation of disease proteins, optogenetic Trim-Away & more! nature.com/articles/s41594-0…
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In @NatureSMB we show how #TrimAway technology works! #TRIM21 molecules must cluster at the target to degrade; #SocialDistancing not good! Embracing this clustering mechanism allows selective degradation of disease proteins, optogenetic Trim-Away & more! nature.com/articles/s41594-0…
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Team effort from Leo James' intracellular immunity lab @MRC_LMB, @Polarity_i3S and @w_mcewan's labs. Looking forward to clustering at the bar one day to celebrate all this hard work!
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Have you seen us in the paper today? Well done to Mrs Walsh for all her hard work setting up Forest School. Y1 have 💕 their session, Y2 next half term 😁
Image from Bewick Bridge CPS (@BewickCps)
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A really clever assay here by @Gui_Pap to quantify cell-cell fusion mediated by SARS-CoV-2 spike protein in real time. Led to some interesting findings.... biorxiv.org/content/10.1101/…
Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion

Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor Ace2 and undergoing virus-host membrane fusion. Fusion is triggered by the protease...

biorxiv.org
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I would recommend this 5 minute read for an introduction to the cytosolic antibody receptor and E3 ubiquiting ligase #TRIM21
Jingwei (@Jingwei_Zeng1) profile pictureJingwei@Jingwei_Zeng17 Aug 2020
A Brief introduction to my PhD topic. #PLOSPathogens: Intracellular antibody immunity and its applications dx.plos.org/10.1371/journal.…
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De novo design of picomolar SARS-CoV-2 miniprotein inhibitors biorxiv.org/cgi/content/shor… #bioRxiv
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#TrimAway. When we published this approach back in 2017, including binder-Fc fusion proteins, someone suggested the name ‘The Triminator’. Is TRIM21 the Arnold Schwarzenegger of proteins?
bioRxiv (@biorxivpreprint) profile picturebioRxiv@biorxivpreprint2 Aug 2020
Predator: A novel method for targeted protein degradation biorxiv.org/cgi/content/shor… #bioRxiv
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Was it you @RossHillScience with The Triminator suggestion?
Engineered ACE2 receptor traps potently neutralize SARS-CoV-2 biorxiv.org/cgi/content/shor… #bioRxiv
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Replying to @DeanClift1
@DeanClift1 and Leo James lab define the mechanism that activates the E3 ligase TRIM21 by clustering. This makes TRIM21 a promising component for #optogenetic control of protein degradation by light-induced clustering. We are happy to have contributed! Filipa and @MarianaOsswald
Dean Clift (@DeanClift1) profile pictureDean Clift@DeanClift130 Jul 2020
TRIM21 uses antibodies to degrade invading pathogens, and any cellular protein #TrimAway, inside cells. Amazing! But how? Here is the fascinating mechanism plus new tools for targeted protein degradation! @MRC_LMB @Polarity_i3S @w_mcewan @MarianaOsswald biorxiv.org/cgi/content/shor…
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Congratulations to our master student Alif for getting the highest grade for his research project on TRIM21’s role in snake toxin neutralization conducted in collaboration with LMB in Cambridge!
Image from Andreas Laustsen (@AndreasLaustsen)
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Very happy to have contributed to this work! 😀 if you are interested in fast and cool ways to degrade proteins check out this pre-print.
Dean Clift (@DeanClift1) profile pictureDean Clift@DeanClift130 Jul 2020
TRIM21 uses antibodies to degrade invading pathogens, and any cellular protein #TrimAway, inside cells. Amazing! But how? Here is the fascinating mechanism plus new tools for targeted protein degradation! @MRC_LMB @Polarity_i3S @w_mcewan @MarianaOsswald biorxiv.org/cgi/content/shor…
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TRIM21 uses antibodies to degrade invading pathogens, and any cellular protein #TrimAway, inside cells. Amazing! But how? Here is the fascinating mechanism plus new tools for targeted protein degradation! @MRC_LMB @Polarity_i3S @w_mcewan @MarianaOsswald biorxiv.org/cgi/content/shor…
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