@NADFDGD

Transcriptional acrobatics. Assistant Professor at the University at Buffalo.

 Buffalo, NY
scholar.google.com/citations?user=w_SIQkwAAAAJ&hl=en&oi=sra
Joined February 2017
  • Tweets1.7K
  • Following746
  • Followers640
238Media
Our latest preprint, led by Skyler Kelly: we developed a platform for high-throughput cotranscriptional RNA biochemistry called Transcription Elongation Complex display. biorxiv.org/content/10.1101/…

Systematic analysis of cotranscriptional RNA folding using transcription elongation complex display

RNA can fold into structures that mediate diverse cellular functions. Understanding how RNA primary sequence directs the formation of functional structures requires methods that can comprehensively...

biorxiv.org
2
6
18
3,738
Our latest preprint "Sequential structure probing of cotranscriptional RNA folding intermediates" is on bioRxiv. biorxiv.org/content/10.1101/… Led by @CourtneySzyjka and anchored by Skyler Kelly, this preprint describes a method for detecting cotranscriptional RNA folding transitions.

Sequential structure probing of cotranscriptional RNA folding intermediates

Cotranscriptional RNA folding pathways typically involve the sequential formation of folding intermediates. Existing methods for cotranscriptional RNA structure probing map the structure of nascent...

biorxiv.org
1
7
1
20
2,076
more replies
And in the presence of ZMP, we also observed both folding transitions and (also as expected) clear signatures of ZMP binding.
1
103
We applied this method to 5 known folding transitions, which allowed us to assess it's generalizability and target RNA-specific challenges that may arise, which are described in detail in the preprint.
82
Eric Strobel retweeted
Taekjip Ha@taekjip21 Mar 2024
RNA deserves its own massive counterpart to the human genome project, researchers argue | Science | AAAS science.org/content/article/…

RNA deserves its own massive counterpart to the Human Genome Project, researchers argue

U.S. report lays out an ambitious plan to harness the “RNome” for medicine and more—but funding is uncertain

science.org
1
28
4
97
11,629
Here is our latest effort. I hope you don't hate it. The translocation activity of Rad54 reduces crossover outcomes during homologous recombination biorxiv.org/content/10.1101/…

The translocation activity of Rad54 reduces crossover outcomes during homologous recombination

Homologous recombination (HR) is a template-based DNA double-strand break repair pathway that requires the selection of an appropriate DNA template for repair during the homology search stage of HR....

biorxiv.org
2
3
2
25
4,354
Eric Strobel retweeted
Tony Mustoe@TonyMustoe10 Jan 2024
Very excited this is out! authors.elsevier.com/a/1iOw0…
2
20
3
79
10,304
Fresh preprint! Cool RecBCD work led by Alessia Lepore in @MEKlab. @ojp25 contributed from my lab. Nice imaging + mechanistic (SOS!) insight. @BBSRC-funded. In vivo single-molecule imaging of RecB reveals efficient repair of DNA damage in Escherichia coli biorxiv.org/content/10.1101/…

In vivo single-molecule imaging of RecB reveals efficient repair of DNA damage in Escherichia coli

Efficient DNA repair is crucial for maintaining genome integrity and ensuring cell survival. In Escherichia coli , RecBCD plays a crucial role in processing DNA ends following a DNA double-strand...

biorxiv.org
5
33
3,327
We have received a lot of requests for help with making streptavidin affinity grids. We are happy to finally share our protocol for robust in-house fabrication of streptavidin affinity grids. Stay tuned for the accompanying video that is coming soon! app.jove.com/t/66197/strepta…
2
99
4
348
49,756
Want to design genetically encoded inhibitors of your favorite protein? Thrilled to announce our latest work w/ @SassSeabass, @keating_lab, & @GeneWeiLiLab, where we leverage massively parallel AlphaFold predictions to discover peptide inhibitors. biorxiv.org/content/10.1101/… (1/n)
11
131
8
522
102,358
Breakfast!
3
294
We applied this to a ZTP riboswitch variant library to investigate the mechanisms by which variants that match the consensus sequence and structure misfold. Panel c illustrates the ZMP-mediated antitermination response for the 32,768 variants in the library!
1
1
175
We designed the TECdisplay platform to be broadly accessible and easy to use, so our hope is that this approach democratizes the development and application of high-throughput RNA biochemical assays.
1
1
140
Load more