Xiang Hao (@ProfHaoX) profile picture

Xiang Hao

@ProfHaoX
Joined March 2018
  • Tweets15
  • Following12
  • Followers14
Aberrations in Structured Illumination Microscopy: A Theoretical Analysis frontiersin.org/article/10.3…
1
1
Fast reconstruction algorithm for structured illumination microscopy osapublishing.org/ol/abstrac…
1
1
For the first time as a corresponding author: Ultra-fast, universal super-resolution radial fluctuations (SRRF) algorithm for live-cell super-resolution microscopy osapublishing.org/oe/abstrac…
1
3
Our first work on Labelling strategy: Labeling subcellular structures in living specimens using live-cell incompatible dyes with excellent optical properties disq.us/t/3olcd1d
1
The best idea I am aware of to find the lens focus in a practical experiment. youtu.be/h5y1h_DWeEM via @YouTube
Just want to know the answer~
Reto Fiolka (@RetoPaul) profile pictureReto Fiolka@RetoPaul24 Feb 2020
Confocal experts: who is credited with the first confocal microscope with a scanning mirror (instead of stage scanning, like Minsky and others)? I reckon Tony Wilson and Colin Sheppard were involved early on, but who put a scanning mirror on it first? CC @HeintzmannLab
Very interesting. The same question has also bothered me for years. Glad to see someone eventually decided to treat it seriously.
Andrew York (@AndrewGYork) profile pictureAndrew York@AndrewGYork13 Feb 2020
Help us resolve a paradox, argue with Einstein, and make fluorescence microscopy a thousand times brighter. Ask your favorite physicist: "What direction does stimulated emission travel from a pointlike emitter?" Our answer: andrewgyork.github.io/stimul… Co-inventor: @sanjay_r_varma
Look Forward to the combination of deep learning and STED! nature.com/articles/s41592-0…
Microscopy enters AI time. cell.com/cell/abstract/S0092…
"#Superresolution microscopy to decipher multi-molecular assemblies" by @Chr_Sieben @kmdouglass @PaulGuichard @SulianaManley - Nice overview which shows that SRM is a valuable addition to the structural biology toolbox. sciencedirect.com/science/ar…
Image from Optical Nanoscopy (@OptNanoscopy)
10
11
Xiang Hao retweeted
HHMI (@hhmi_science) profile picture
HHMI
@hhmi_science30 Mar 2018
What is expansion microscopy and how does it work? Click to expand. bit.ly/2J6HbqQ
1. Apply chemical agent to tissue to preserve cell structure & any fluorescent proteins. Add fluorescent antibodies to detect proteins of interest. 2. Add Acryloyl-X which binds to the tissue's lysines & anchors them to the gel as it forms. 3. Form gel matrix throughout sample & add proteinase-K which digests proteins into fragments so they are able to expand. 4. Add water which washes out salt to drive expansion.

ALT 1. Apply chemical agent to tissue to preserve cell structure & any fluorescent proteins. Add fluorescent antibodies to detect proteins of interest. 2. Add Acryloyl-X which binds to the tissue's lysines & anchors them to the gel as it forms. 3. Form gel matrix throughout sample & add proteinase-K which digests proteins into fragments so they are able to expand. 4. Add water which washes out salt to drive expansion.

7
1
13
Using immersion objective at low temperature. Nice work! teapaw.org/OptNanoscopy/status/97…
Optical Nanoscopy (@OptNanoscopy) profile pictureOptical Nanoscopy@OptNanoscopy26 Mar 2018
"Aberration-corrected cryoimmersion light microscopy" by Faoro et al. @PNASNews #nanoscopy #superresolution pnas.org/content/115/6/1204
Image from Optical Nanoscopy (@OptNanoscopy)
SMS + DNA-PAINT + line-scan confocal teapaw.org/SRMicro_papers/status/…
Super Res Microscopy (@SRMicro_papers) profile pictureSuper Res Microscopy@SRMicro_papers17 Mar 2018
Superresolution fluorescence microscopy for 3D reconstruction of thick samples. by Park S, Kang W, Kwon YD, Shim J, Kim S, Kaang BK, Hohng S ift.tt/2DAFQo1
Expansion stimulated emission depletion microscopy (ExSTED) disq.us/t/3014mmg nice work from @ewers_helge's lab
Load more