We develop technologies @harvardmed that combine #stem_cell biology, #CRISPR_Cas9 genome editing screens, and #computational_biology to understand gene function
Brigham and Women's Hospital
Joined March 2020
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(1/8) Excited to share our latest preprint introducing ACCESS-ATAC, a high-resolution approach to measure chromatin accessibility and transcription factor binding: biorxiv.org/content/10.1101/…
LDLR variants are the most common cause of inherited high cholesterol, but we don’t know which are harmful. We used prime editing to measure >5,000, out now in @CircAHA, led by @phillipzhou24, Minja Velimirovic & @TiannnYuu with @CassaLabBWH 🧵
ahajournals.org/doi/10.1161/… (1/15)
Huge thanks to the team that made this possible: @glig_v, Nicolas Mathis, Jing Zhao, Quang Vinh Phan, Felicitas Vogd, @JayoungR, Qisheng Pan, Atharva Tyagi, @DrDavidAscher, @schwanklab, @lucapinello, @ccassa and others. (14/15)
Want to work through the analysis yourself? We made a demo for Harvard’s genetics bootcamp: (15/15)
colab.research.google.com/gi…
(7/8) In sum, ACCESS-ATAC improves the resolution of ATAC-seq and opens up a new ability to analyze single-allele TF co-occupancy. Want to try it? Reach out-- we have built flexible experimental and computational workflows and would be happy to share reagents and expertise!
(8/8) Plus we’re not the only ones who have realized the power of Ddd enzymes in chromatin analysis: check out the work from @brian_b_liau and colleagues combining DddA and CRISPR to dissect how sequence impacts chromatin state: biorxiv.org/cgi/content/shor…