Puneet juneja (@chattbuzz) profile picture

Puneet juneja

@chattbuzz

Structural Biochemist @Thermo Fisher @Novavax

 Maryland, USA
Joined May 2011
  • Tweets81
  • Following179
  • Followers107
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Photo by Puneet juneja (@chattbuzz)Photo by Puneet juneja (@chattbuzz)Photo by Puneet juneja (@chattbuzz)Photo by Puneet juneja (@chattbuzz)Photo by Puneet juneja (@chattbuzz)
Scientists from @stonechenlab, @ezraburstein, @Turer_Lab,@CryoEM_ISU report insights into the function of Sorting Nexin 17 (SNX17) and Retriver during endosomal recycling @gdcbisu, @bbmbisu, @chattbuzz nature.com/articles/s41467-0…
Image from Nature Communications (@NatureComms)
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Rafa F Leiro (@rfleiro) profile picture
Rafa F Leiro@rfleiro14 Jul 2022
Hi #cryoEM! @Nayimcete and I have made some tools that we find quite useful for cryoEM data processing at different stages. Have a look. We hope you find them useful! If you feel like contributing, please go ahead. That would be awesome! Any feedback would be very welcomed too!
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Excited to discuss our new preprint that has been a work in progress for the past two years! TL;DR – we propose a new framework for #cryoEM data collection that makes decisions and plans a path across cryo-EM grids biorxiv.org/content/10.1101/… Thread 👇
Optimized path planning surpasses human efficiency in cryo-EM imaging

Optimized path planning surpasses human efficiency in cryo-EM imaging

Cryo-electron microscopy (cryo-EM) represents a powerful technology for determining atomic models of biological macromolecules[1][1]. Despite this promise, human-guided cryo-EM data collection...

biorxiv.org
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Dipa Sashital (@dsashital) profile picture
Dipa Sashital@dsashital31 May 2022
And for our second #CRISPR preprint of the day: The first cryo-EM structures from the @CryoEM_ISU facility! Yukti @yukti__dhingra, Shravanti @shra_krishna, and Puneet @chattbuzz report cryo-EM structures and biochemistry of the type I-C Cas4-Cas1-Cas2 complex! Thread...
Image from Dipa Sashital (@dsashital)
bioRxiv (@biorxivpreprint) profile picturebioRxiv@biorxivpreprint31 May 2022
PAM binding ensures orientational integration during Cas4-Cas1-Cas2 mediated CRISPR adaptation biorxiv.org/cgi/content/shor… #bioRxiv
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Happy to report this Cas4 story with @shra_krishna and @sashital demonstrating how Cas4-Cas1-Cas2 updates the CRISPR array with new spacers in type I-C systems! Been an exciting journey towards the first cryoEM story from @CryoEM_ISU and working alongside Puneet @chattbuzz
Dipa Sashital (@dsashital) profile pictureDipa Sashital@dsashital31 May 2022
And for our second #CRISPR preprint of the day: The first cryo-EM structures from the @CryoEM_ISU facility! Yukti @yukti__dhingra, Shravanti @shra_krishna, and Puneet @chattbuzz report cryo-EM structures and biochemistry of the type I-C Cas4-Cas1-Cas2 complex! Thread...
Image from Dipa Sashital (@dsashital)
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We are expanding the @UWBiochem CEMRC and the #NIH National CryoET Center! Interested in #CryoEM, #teamtomo, #CLEM, and working with a great #microscopy team? Check out the scientist advert! | Also great #Postdoc posts | DM & RT jobs.hr.wisc.edu/en-us/job/5…
Image from Elizabeth Wright (@erwright73)
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Nick Schnicker (@n_schnicker) profile picture
Nick Schnicker@n_schnicker2 Sep 2021
Screening #cryoEM grids at the @IowaStateU CryoEM Facility 🧊🔬 #UIowaResearch @PCF_uiowa @IowaMed
Image from Nick Schnicker (@n_schnicker)
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Cell Press (@CellPressNews) profile picture
Cell Press@CellPressNews6 Aug 2021
Online now: "No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing" - Read the Report from @Faulkner_Lab and colleagues in @CellReports hubs.ly/H0TDHYM0
Image from Cell Press (@CellPressNews)
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Oded Rechavi (@OdedRechavi) profile picture
Oded Rechavi
@OdedRechavi18 Jun 2021
Manuscripts are assessed the same no matter where the authors are from
Image from Oded Rechavi (@OdedRechavi)
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Carter Lab (@Carter_Lab) profile picture
Carter Lab@Carter_Lab18 Jun 2021
Is this the start of the resolution revolution in AFM? #journalclub doi.org/10.1038/s41586-021-0…
Image from Carter Lab (@Carter_Lab)
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Interested in working in #structuralbiology at the interface between #CryoEM & #xray ? We are expanding our team @UWBiochem. Check out the advert! jobs.hr.wisc.edu/en-us/job/5… | DM & RT
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Diamond ILO (@DiamondILO) profile picture
Diamond ILO@DiamondILO22 Apr 2021
Join the eBIC team @DiamondLightSou for this practical #cryoEM course, 26-29 April: ‘Electron Cryo Microscopy in Structural Biology’ covering topics from sample prep through to image processing, reconstruction & modelling. Open to attendees from industry: loom.ly/UsnZCOk
Image from Diamond ILO (@DiamondILO)
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A Further Turn of the Screw chemistrycommunity.nature.co… A Behind the Paper story on our recent Nature Communications article @ManuelaOrdy @jerrynosnothing @conticellolab @EmoryChem
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Synthetic shape-shifting collagen with potential biomedical applications - Materials Today materialstoday.com/biomateri… A nice summary of our recently published research in Materials Today. @conticellolab @EmoryChem
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Breaking the next Cryo-EM resolution barrier - Atomic resolution determination of proteins! biorxiv.org/cgi/content/shor…
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Ellen Zhong (@ZhongingAlong) profile picture
Ellen Zhong
@ZhongingAlong30 Mar 2020
Release the cryoDRGN ❄️🐉 (on biorxiv)! Instead of static protein structures, we develop a neural network architecture to reconstruct diverse conformations and molecular motions from real cryo-EM image data. Joint work with Tristan Bepler, Bonnie Berger, and @jhdavislab.
cryoEM Papers (@cryoEM_Papers) profile picturecryoEM Papers@cryoEM_Papers29 Mar 2020
CryoDRGN: Reconstruction of heterogeneous structures from cryo-electron micrographs using neural networks biorxiv.org/cgi/content/shor…
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At noon today, the Department of Public Health released updated numbers for positive COVID-19 cases in Georgia (dph.georgia.gov/covid-19-dai…). As widely reported last night, we have two confirmed cases in Athens.
Image from Houston Gaines (@houstongaines)
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Replying to @mcianfro @annotated_sci
These beam-image shifts definitely introduced aberrations. We could only get a structure of aldolase to 5.6Å initially (!). But, after repeated CTF refinement and particle polishing in #RELION, we could increase the resolution from 5.6Å to 2.8Å. Very impressive #RELION!
Image from Michael Cianfrocco (@mcianfro)
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Tamir Gonen (@gonenlab) profile picture
Tamir Gonen@gonenlab13 Nov 2019
Everyone boasting structure in 2 days; structure in 30min (which we are guilty of also). But, people, whats the rush? Stop and smell the roses. Enjoy the process. Learn cryoem. Learn crystallography. Dont just learn to press a button. Theres too much to miss by rushing. Have fun.
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His solution “belongs in a Star Trek episode, it is that cool!” says Eva Nogales @UCBerkeley @BerkeleyLab in Holger Müller profile rdcu.be/bR3AZ  Laser phase plate to improve contrast in #cryoEM. Paper: rdcu.be/bS1Wh N&V by @RadoDanev rdcu.be/bS1Ws
Image from Nature Methods (@naturemethods)
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